低温对心搏骤停大鼠心肺复苏后海马CRIP表达的影响  

Effect of hypothermia on expression of cold-inducible RNA-binding protein after cardiopuimonary resuscitation in a rat model of cardiac arrest

作　　者：吴淋 高宇[1] 惠康丽[1] 蔡慎权 范婧婧[1] 仲浩[2] 徐苗苗[1] 段满林[1] 徐建国[1] 

机构地区：[1]南京军区南京总医院麻醉科,210002 [2]徐州医学院江苏省麻醉学重点实验室,221004

出　　处：《中华麻醉学杂志》2016年第2期235-238,共4页Chinese Journal of Anesthesiology

基　　金：天普研究基金（UF201407）;江苏省麻醉学重点实验室开放研究课题（KJS1402）

摘　　要：目的评价低温对心搏骤停大鼠心肺复苏后海马冷诱导RNA结合蛋白（CIRP）表达的影响。方法清洁级健康雄性SD大鼠90只，体重280～320g，采用随机数字表法分为3组（n=30）：假手术组（Sham组）、心搏骤停-心肺复苏组（CPR组）和低温组（H组）。Sham组只进行动静脉穿刺，气管插管等手术操作，不给予电刺激和心肺复苏；CPR组经食管电击致心搏骤停并行心肺复苏；H组于自主循环恢复（ROSC）即刻，将大鼠置于冰袋上行体表降温，15min内将直肠温度降至32～34℃，平稳维持6h，其余组维持直肠温度36～37℃。分别于ROSC后24和72h时行神经功能评分，随后处死大鼠取海马，HE染色观察海马CA1区锥体细胞病理学结果；荧光实时PCR法检测CIRPmRNA表达；Western blot法检测CIRP表达。结果与Sham组比较，CPR组和H组ROSC后24和72h时神经功能评分降低，CIRP及其mRNA表达上调（P〈0．05）；与CPR组比较，H组ROSC后72h时神经功能评分升高，ROSC后24和72h时CIRP及其mRNA表达上调（P〈0．05）。结论低温可有效减轻心搏骤停大鼠心肺复苏后脑缺血再灌注损伤，其机制可能与上调CIRP表达有关。Objective To evaluate the effect of hypothermia on the expression of cold-inducible RNA-binding protein （CIRP） after cardiopulmonary resuscitation （CPR） in a rat model of cardiac arrest. Methods Ninety pathogen-free male Sprague-Dawley rats, weighing 280-320 g, were randomly divided into 3 groups （n=30 each） using a random number table： sham operation group （group Sham） , cardiac arrest-CPR group （group CPR） , and hypothermia group （group H）. Group Sham underwent only cannula- tion of the artery and vein and tracheal intubation. In group CPR, cardiac arrest was induced by electric stimulation via the esophagus, and then CPR was performed. In group H, the rectal temperature was cooled down to 32-34 ℃ within 15 min by putting rats on the ice bags immediately after recovery of spontaneous circulation （ROSC） , and maintained at this level for 6 h, while the rectal temperature was maintained at 36-37℃ in the other groups. Neurological deficit score （NDS） was evaluated at 24 and 72 h after ROSC, and the rats were then sacrificed. The hippocampus was removed for examination of the pathological changes of pyramidal cells in the hippocampal CA1 region （using hematoxylin and eosin staining） , and for determination of the expression of CIRP mRNA and protein （using fluorescent quantitative real-time reverse transcriptase-polymerase chain reaction or Western blot）. Results Compared with group Sham, NDS score was significantly decreased, and the expression of CIRP mRNA and protein was up-regulated at 24 and 72 h after ROSC in CPR and H groups （ P〈0.05）. Compared with group CPR, NDS score was significantly increased at 72 h after ROSC, and the expression of CIRP mRNA and protein was up-regulated at 24 and 72 h after ROSC in group H （P〈0.05）. Conclusion Hypothermia can effectively reduce cerebral ischemia-reperfusion injury and improve neurological function after CPR, and the mechanism is probably related to up-regulation of CIRP expression in a rat model of cardiac arres

关 键 词：低温 人工 心脏停搏 人工 心肺复苏术 载体蛋白质类 海马 

分 类 号：R541.78[医药卫生—心血管疾病]