低强度振动载荷对兔长骨再生速率的影响  

Effects of low-level vibration load on bone regeneration rate of rabbit long bone

作　　者：佟世超[1,2] 唐振宏 刘娟[1] 申广浩[1] 翟明明[1] 景达[1] 李小康[4] 郭征[4] 罗二平[1] 

机构地区：[1]第四军医大学生物医学工程学院,陕西西安710032 [2]第230医院,辽宁丹东118000 [3]陕西省医疗器械检测中心,陕西西安710032 [4]第四军医大学西京医院骨科,陕西西安710032

出　　处：《中国医学物理学杂志》2016年第4期364-367,共4页Chinese Journal of Medical Physics

基　　金：国家自然科学基金(81471806)

摘　　要：目的:比较低强度全身振动刺激(WBV)协同多孔钛合金(pTi)与单独pTi对骨缺损的修复效果的差异。方法:Ti-6Al-4V粉末制成的圆柱体多孔填充材料植入雌性新西兰白兔股骨外侧髁中。将实验动物按体质量随机分为pTi组(对照组)和pTi+WBV组,每组12只。pTi+WBV组于术后1周开始每天施加频率30 Hz、加速度0.5 g的垂直振动刺激1 h。取材前14天及前4天肌肉注射钙黄绿素。WBV刺激后6、12周取材,定量比较pTi中的骨小梁矿化沉积率以及骨小梁表面的成骨细胞数量。结果:(1)pTi+WBV组6、12周骨缺损部位骨小梁生长速率明显高于对照组(P<0.05);(2)pTi+WBV组成骨细胞数量明显高于对照组。结论:WBV在骨缺损治疗中与pTi有协同作用,WBV能够促进骨小梁与钛合金的整合,并能够提高体内骨形成活性。Objective To compare the repair effects of low-level whole body vibration（WBV） combined with porous titanium alloy（pTi） and pTi alone on bone defects. Methods Porous cylinder filling materials made of Ti-6Al-4V power were implanted into the lateral femur condyle of female New Zealand white rabbits which were randomly divided into the pTi group and pTi＋WBV group according to the weight, each group of 12 rabbits. The rabbits in pTi＋WBV group had been subjected to 1 h/d vertical WBV stimulation with frequency of 30 Hz and acceleration of 0.5 g since one week after the operation. All rabbits received intramuscular injections of 8 mg/kg calcein on 14 and 4 days before sacrifice. After WBV stimulation for 6 and 12 weeks, rabbits in each group were euthanatized for quantitatively comparing the mineral apposition rate of bone trabecula and the number of osteoblast on the surface of bone trabecula. Results After the WBV stimulation for 6 and 12 weeks, the bone trabecula regeneration rate of bone defects in pTi＋WBV group was significantly higher than that in control group（P〈0.05）. And the number of osteoblast in pTi＋WBV group was significantly higher than that in control group. Conclusion WBV and pTi has the cooperation on the treatment of bone defects. WBV can promote integration of pTi and bone trabecula, and enhance bone formation activity in vivo.

关 键 词：骨缺损 骨再生 多孔钛合金 全身振动刺激 骨矿化沉积率 成骨细胞 

分 类 号：R683.42[医药卫生—骨科学]