调控人骨桥蛋白的miRNA预测研究  

Study of the prediction of micro RNA regulating human osteopontin

作　　者：苏里[1] 赵志军[1] 梅小龙[1] 杨建华[1] 包文平[1] 张春阳[1] 

机构地区：[1]包头医学院第一附属医院神经外科,内蒙古包头014010

出　　处：《中国临床神经外科杂志》2016年第4期210-212,共3页Chinese Journal of Clinical Neurosurgery

基　　金：国家自然科学基金项目(81360164);内蒙古自然科学基金项目(2014MS0806)

摘　　要：目的利用生物信息学方法预测调控人骨桥蛋白的微小RNA(miRNA),为进一步实验验证特定miRNA和人骨桥蛋白基因之间的关系提供依据。方法分别应用miRGen Targets和miRanda数据库确定与人骨桥蛋白有密切关系的miRNA分子。结果经miRGen Targets数据库预测出28个miRNA分子可能作用于人骨桥蛋白基因;miRanda数据库显示miR-539,miR-181,miR-340,miR-376c和miR-154 5个miRNAs在人骨桥蛋白的负调控过程可能发挥作用;将两个数据库结果交集后发现miR-539,miR-181,miR-33b和miR-377 4个miRNA分子最有可能参与调控人骨桥蛋白。结论利用生物信息学软件可对调控人骨桥蛋白的miRNA进行预测,为研究骨桥蛋白的功能和生物学特性提供新手段。Objective To predict some micro RNAs（miRNA） regulating human osteopontin（OPN） with bioinformatic softwares in order to provide a valid theoretical support for further experimental research on the relationship between specific miRNA and human OPN gene.Methods Integrated bioinformatic database including miRGen Target and miRanda were respectively applied to identify the number of miRNAs which closely related with human OPN.Results The miRGen Target database showed that 28 miRNAs might be involved in the degradation of OPN and miRanda database showed that 4 miRNAs might play an important role in the process of the negative regulation of OPN. The intersection of two database found that miR-539, miR-181, miR-33 b and miR-377 were most likely involved in regulation of human OPN.Conclusion The prediction of miRNA regulating human OPN with bioinformatical methods can provide a new strategy for the study of the property as well as function of OPN.

关 键 词：微小RNA 骨桥蛋白 生物信息学 靶基因预测 

分 类 号：Q786[生物学—分子生物学]