THBS3基因在长白猪和通城猪不同组织和不同时期骨骼肌中的表达分析  

Expression Analysis of THBS3 Gene in Different Tissues and Skeletal Muscles at Different Periods from Landrace and Tongcheng Pigs

作　　者：马喜山[1] 唐中林[2] 肖冲[1] 杨博超[1] 秦川[1] 

机构地区：[1]中国医学科学院医学实验动物研究所,北京100021 [2]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《中国畜牧兽医》2016年第4期1032-1038,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31201787)

摘　　要：本研究主要探究血小板反应蛋白3(thrombospondin-3,THBS3)基因在不同组织及不同时期骨骼肌生长发育过程中的表达规律。利用实时荧光定量PCR方法对THBS3基因在长白猪和通城猪中的组织表达谱,以及在胚胎期(33、45、65、70和90d)和出生后阶段(0、9、30、60、120和160d)骨骼肌中的差异表达进行了比较分析。结果表明,THBS3基因广泛表达于长白猪和通城猪各个组织器官,除胃和肠中的表达存在差异外,其在两个猪种中的组织表达谱基本一致,在肺脏中表达量最高。THBS3基因在长白猪和通城猪胚胎期骨骼肌中的表达水平均显著高于出生后阶段(P<0.05),但胚胎期骨骼肌中的表达模式在两个猪种间存在一定差异,THBS3基因表达峰值出现在长白猪胚胎期45d,而其在通城猪中表达峰值出现在胚胎期65d。结果提示,THBS3基因参与了猪骨骼肌生长发育过程及不同类型猪种骨骼肌生长发育异步性的调控。To study the expression pattern of THBS3 gene in different tissues and during skeletal muscle development,the THBS3 gene expression in different tissues and skeletal muscles during prenatal periods（33,45,65,70 and 90d）and postnatal periods（0,9,30,60,120 and 160d）from Landrace and Tongcheng pigs were detected by Real-time quantification PCR.The results showed that THBS3 gene widely expressed in all tissues examined,exhibiting similar spatial expression patterns with expression peaks in lung in both pig breeds except in stomach and intestine.Moreover,although THBS3 gene showed a significant higher expression level in gestation than after birth in Landrace and Tongcheng pigs（P〈0.05）,it exhibited different expression patterns between Landrace and Tongcheng pigs,the expression peak was detected at gestation day 45 in Landrace pig,while was detected at gestation day 65 in Tongcheng pig.The results suggested that THBS3 gene involved in skeletal muscle growth and development in pigs,as well as the regulation of asynchronization of skeletal muscle development in different pig breeds.

关 键 词：猪 THBS3基因 骨骼肌 实时荧光定量PCR 表达 

分 类 号：Q786[生物学—分子生物学]