肝硬化患者红细胞膜抗溶性增强对淋巴细胞亚群检测的干扰及纠正  

Interference and correction of erythrocyte membrane anti-hemolytic enhancement in patients with liver cirrhosis on lymphocyte subset detection

作　　者：何东元[1] 魏崇莉[2] 柴翠翠 张文婷[1] 周文慧[1] 李丽[1] 苟莹[1] 

机构地区：[1]中国人民解放军第一医院输血科,兰州730000 [2]兰州军区机关门诊部,兰州730000

出　　处：《检验医学与临床》2016年第8期1035-1036,1039,共3页Laboratory Medicine and Clinic

摘　　要：目的探讨肝硬化患者标本抗溶性增强条件下淋巴细胞亚群检测时标本制备的最佳溶血条件。方法用不同剂量的溶血素在不同溶血时间处理34例抗溶性增强的肝硬化患者标本,观察在不同溶血条件下流式细胞仪获取的前向散射光(FSC)和侧向散射光(SSC)下白细胞散点图的清晰程度,同时测定T淋巴细胞、B淋巴细胞和自然杀伤(NK)细胞的百分比。结果肝硬化患者红细胞膜抗溶性增强,采用溶血素用量为1 350μL、溶血时间为10min淋巴细胞分群效果较好。结论制备肝硬化患者淋巴细胞亚群标本时,增加溶血素用量,检测结果的准确性增强。Objective To explore the best hemolysis condition of specimen preparation under the condition of specimen anti-hemolysis increase in the patients with liver cirrhosis.Methods Different doses of hemolysin were used to treat the anti-hemolysis enhanced specimens from 34 cases of liver cirrhosis in different hemolytic time.The clear degree of WBC scatter diagram under FSC and SSC obtained by cytometry was observed under different hemolytic conditions.Meanwhile the percentages of T,B and NK lymphocyte subsets were detected.Results The anti-hemolysis of erythrocyte membrane in the patients with liver cirrhosis was enhanced.When hemolysin amount was1 350μL and the hemolytic time was 10 min,the classification results of lymphocyte subsets were better.Conclusion In preparing the lymphocyte subset specimen in the patients with liver cirrhosis,increasing the hemolysin dosage enhances the accuracy of detection results.

关 键 词：肝硬化 淋巴细胞亚群 溶血素 流式细胞术 

分 类 号：R758.710.4[医药卫生—皮肤病学与性病学]