胃癌干细胞CSC-G在胃癌侵袭和转移中的作用  

Effect of gastric cancer stem cells CSC-G on invasion and metastasis of gastric cancer cells

作　　者：李向辉[1] 王贵吉[1] 

机构地区：[1]郑州大学第一附属医院肿瘤科三病区,河南省郑州市450052

出　　处：《中国组织工程研究》2016年第11期1597-1602,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81170520)~~

摘　　要：背景:研究表明肿瘤干细胞在肿瘤的侵袭和转移中发挥重要作用,关于胃癌干细胞在胃癌的侵袭和转移中的作用的研究不多。目的:观察胃癌干细胞CSC-G在胃癌侵袭和转移中的作用。方法:对胃癌干细胞和SGC7901胃癌细胞进行为期10 d的体外培养。进行球形克隆形成实验,Western blot法检测胃癌干细胞和SGC7901胃癌细胞的OCT4,SOX2,E-cadherin和CD44蛋白表达,Transwell法检测胃癌干细胞和SGC7901胃癌细胞的侵袭性和迁移能力。结果与结论:1细胞形态:胃癌细胞SGC7901在RPMI1640培养基中呈现贴壁生长,传代后细胞为四边形或者多角形;胃癌干细胞CSC-G在无血清培养基中呈悬浮生长,贴壁生长的胃癌干细胞呈梭形或圆形;2细胞侵袭性和迁移性:与胃癌细胞SGC7901相比,胃癌干细胞CSC-G的OCT4,SOX2,CD44蛋白表达:瘤球数量,穿过基底膜的细胞数明显升高(P<0.05),E-cadherin蛋白表达降低(P<0.05);3结果表明,实验成功体外培养了胃癌干细胞CSC-G,其侵袭性和迁移能力与胃癌细胞相比明显增强,说明胃癌干细胞CSC-G在胃癌侵袭和转移中发挥重要作用。BACKGROUND： Studies have shown that cancer stem cells play an important role in tumor invasion and metastasis, but studies on the role of gastric cancer stem cells in invasion and metastasis of gastric cancer cells were rarely reported.OBJECTIVE： To study the effect of gastric cancer stem cells CSC-G on invasion and metastasis of gastric cancer cells. METHODS： Gastric cancer stem cells CSC-G and gastric cancer cells SGC7901 were cultured in vitro for 10 days followed by spherical colony formation assay, western blot assay for detecting OCT4, SOX2, E-cadherin and CD44 protein expression levels in gastric cancer stem cells CSC-G and gastric cancer cells SGC7901, and Transwell assay for detecting the invasion and migration of gastric cancer stem cells and gastric cancer cells SGC7901. RESULTS AND CONCLUSION： Gastric cancer cells SGC7901 in RPMI1640 medium presented with adherent growth and were quadrilateral or polygonal after passage; gastric cancer stem cells CSC-G in serum-free medium presented with suspension growth, and adherent gastric cancer stem cells were spindle-shaped or round. Compared with gastric cancer cells SGC7901, the protein expressions of OCT4, SOX2 and CD44, the number of cancer cell spheres and the number of trans-membrane cells were significantly increased in the gastric cancer stem cells CSC-G（P〈0.05）, and the expression of E-cadherin protein was significantly decreased（P〈0.05）. These findings indicate that the gastric cancer stem cells CSC-G can be successfully cultured in vitro, and have enhanced invasion and migration compared with the gastric cancer cells SGC7901, which play an important role in gastric cancer invasion and metastasis.

关 键 词：组织工程 胃肿瘤 干细胞 组织构建 胃癌 胃癌干细胞 更新 分化 侵袭 转移 复发 球形克隆无血清培养 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]