复合补片和生物补片修复犬腹壁缺损的对比  

Composite patch versus biological patch in the repair of canine abdominal wall defects

作　　者：段友良[1] 林奥[2] 

机构地区：[1]山东省交通医院,山东省济南市250031 [2]山东大学医学院人体解剖学基础实验室,山东省济南市250012

出　　处：《中国组织工程研究》2016年第12期1753-1758,共6页Chinese Journal of Tissue Engineering Research

基　　金：山东卫生科技发展项目(2012AA1202001)~~

摘　　要：背景:目前对于复合补片修复腹壁缺损的效果存在很大争议。目的:对比复合补片和生物补片修复犬腹壁缺损的效果。方法:取10只犬,制作腹壁缺损模型后随机分组,实验组采用复合补片进行修复,对照组采用生物补片进行修复。修复后30,60,90 d,评估切口粘连情况;修复后14 d,取补片及腹壁缺损组织进行组织学检测。结果与结论:实验组不同时间点的切口粘连轻于对照组(P<0.05)。修复后14 d,实验组材料结构清晰,无吸收征象,材料周围存在大量炎性细胞浸润,如中性粒细胞、淋巴细胞等,无细胞吞噬补片;对照组材料结构模糊,存在炎性细胞浸润,出现轻微吸收征象,且炎性反应、纤维增生情况重于实验组。结果表明,与生物补片相比,复合补片修复腹壁缺损可有效减轻炎性反应。BACKGROUND： Currently a big controversy erupts in the repair of abdominal wall defects with composite patch. OBJECTIVE： To compare the composite patch and biological patch in the repair of abdominal wall defects in dogs. METHODS： Ten dogs were selected to prepare abdominal wall defect models, and then randomized into experimental group subjected to composite patch repair and control group subjected to biological patch repair. Incision adhesion was assessed at postoperative 30, 60, 90 days; patches and defect tissues were taken and detected histologically at postoperative 14 days. RESULTS AND CONCLUSION： The incision adhesion in the experimental group was milder than that in the control group at different time post operation（P〈0.05）. At 14 days post operation, the material structure was clear with no signs of absorption in the experimental group, and there were a large amount of infiltrated inflammatory cells around the patch material, such as neutrophils and lymphocytes, and non-phagocytic patch was found; in the control group, the material structure was fuzzy with the presence of inflammatory cell infiltration and slight signs of absorption. Moreover, inflammation and fibrosis were severer in the control group than the experimental group. These findings indicate that compared with the biological patch, the composite patch is better to effectively reduce inflammatory reactions.

关 键 词：腹壁 壳聚糖 组织工程 生物材料 材料相容性 复合补片 生物补片材料 雄性普通犬 修复效果 实验性 腹部缺损模型 吸收征象 应用价值 

分 类 号：R318[医药卫生—生物医学工程]