钛表面梯度生物活性涂层材料在颅骨修补中的生物性能  

Biological properties of gradient bioactive coatings on titanium surface in the skull repair

作　　者：黄俊红[1] 叶党华[1] 桂志勇[1] 谭占国[1] 孟玉丽[2] 

机构地区：[1]漯河医学高等专科学校第一附属医院神经外二科,河南省漯河市462000 [2]漯河医学高等专科学校第一附属医院肝病科,河南省漯河市462000

出　　处：《中国组织工程研究》2016年第12期1772-1778,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:钛网修补颅骨缺损具有良好的临床效果,但由于钛网缺乏骨诱导能力,与骨组织的结合力较差。目的:观察钛表面梯度生物活性涂层材料在颅骨修补中的生物性能。方法:将含梯度生物活性涂层钛网与纯钛网分别与成骨细胞共培养14 d,采用MTT法检测细胞增殖。纳入71例颅骨缺损患者,其中男43例,女28例,年龄15-60岁,分2组修复,观察组(n=33)采用含梯度生物活性涂层钛网进行修补,对照组(n=38)采用纯钛网进行修补。修补后随访12个月,观察修复效果及不良反应。结果与结论:(1)体外实验结果:活性涂层钛网组培养8,10,12,14 d的细胞增殖活性显著高于纯钛网组(P<0.05)。(2)体内修补试验结果:观察组术后住院天数、伤口愈合时间显著短于对照组(P<0.05),末次随访修复效果满意率显著高于对照组(P<0.05),两组钛网固定牢固,未出现浮动,未出现感染及修补材料外露。(3)结果表明,钛网表面的梯度生物活性涂层具有良好的生物相容性及促成骨性能。BACKGROUND： Titanium mesh has good clinical effect in repairing skull defects, but due to the lack of bone induction ability, the titanium mesh has a poor integration with the bone tissue. OBJECTIVE： To observe the biological properties of the gradient bioactive coating materials on the titanium surface in the skull repair. METHODS： Osteoblasts were co-cultured with the titanium mesh with or without gradient bioactive coatings for 14 days, and then cell proliferation was detected using MTT method. Seventy-one patients with skull defects were enrolled, including 43 males and 28 females, aged 15-60 years, and were subjected to skull repair using the titanium mesh with（observation group, n=3） or without（control group, n=38） gradient bioactive coatings. During the postoperative follow-up of 12 months, the repairing effects and adverse reactions were observed in the two groups. RESULTS AND CONCLUSION：（1） In vitro cell culture： the cell proliferative ability was increased significantly in the observation group as compared with the control group at 8, 10, 12 and 14 days after cell culture.（2） In vivo repair： the hospital stay and wound healing time in the observation group were significantly lower than those in the control group（P〈0.05）, and at the final follow-up, the postoperative recovery effect was significantly higher in the observation group than the control group（P〈0.05）. The titanium meshes were fixed firmly in the two groups, with no floating, infection and exposure. These results show that the titanium mesh with gradient bioactive coating has good biocompatibility and osteoinduction capacity.

关 键 词：钛 颅骨 硬羟基磷灰石 组织工程 生物材料 材料相容性 钛金属 颅骨缺损 颅骨修补材料 梯度涂层 羟基磷灰石 生物相容性 生物性能 

分 类 号：R318[医药卫生—生物医学工程]