鞘内注射Caspase-6抑制剂Z-VEID-FMK对小鼠福尔马林炎性痛的影响  

Effect of intrathecal Caspase-6 inhibitor Z-VEID-FMK on mice with formalin inflammatory pain

作　　者：殷智宇[1] 楚帅帅 孙青[1] 李璐[2] 顾小萍[2] 马正良[2] 

机构地区：[1]徐州医学院江苏省麻醉学重点实验室江苏省麻醉与镇痛应用技术重点实验室,江苏徐州221000 [2]南京大学医学院附属鼓楼医院麻醉科,江苏南京210000

出　　处：《中国医药导报》2016年第9期31-34,共4页China Medical Herald

基　　金：江苏省医学重点学科项目(XK201140)

摘　　要：目的研究鞘内注射Caspase-6抑制剂Z-VEID-FMK对小鼠福尔马林炎性痛的影响。方法采用小鼠右后足底皮内注射5%福尔马林溶液建立疼痛模型,32只小鼠根据随机数字表法分成四组:空白组、溶媒组、低剂量组和高剂量组,每组8只。空白组仅建立疼痛模型,溶媒组、低剂量组和高剂量组分别鞘内注射20%二甲基亚砜(DMSO)、5μg Z-VEID-FMK和10μg Z-VEID-FMK,给药体积为5μL,随后建立疼痛模型。观察福尔马林炎性痛小鼠舔/咬脚时间,比较模型建立前及建立后60 min小鼠足底的厚度。结果各组小鼠足底皮内注射福尔马林后均出现明显的双时相疼痛反应,在一相痛中,低剂量组[(88.88±6.98)s]、高剂量组[(90.09±8.49)s]与溶媒组[(90.50±11.18)s]小鼠舔/咬脚时间比较,差异无统计学意义(P>0.05);在二相痛中,与溶媒组[(220.25±80.80)s]比较,低剂量组[(81.25±14.10)s]、高剂量组[(50.63±10.77)s]小鼠舔/咬脚时间均显著缩短,差异有高度统计学意义(P<0.01)。与溶媒组[(1.78±0.02)mm]比较,低剂量组[(1.31±0.07)mm]及高剂量组[(1.11±0.08)mm]小鼠足底肿胀显著降低,差异有高度统计学意义(P<0.01)。结论 Caspase-6抑制剂Z-VEID-FMK可以缓解小鼠福尔马林炎性痛,可能与其抑制炎性因子作用有关。Objective To investigate the effect of intrathecal injection Caspase-6 inhibitor Z-VEID-FMK on mice with formalin inflammatory pain. Methods The pain model was established by subcutaneous injection 5% formalin in mouse′ right plantar. 32 mice were divided into four groups： control group, vehicle group, low-dose group, high-dose group according to random number table method, each group had 8 mice. Pain model was established only in control group. Vehicle group, low-dose group and high dose group was intrathecal injected 20% DMSO, 5 μg Z-VEID-FMK and 10 μg Z-VEID-FMK（the volume was 5 μL） respectively, followed by the establishment of pain model. The mice licking/biting foot time with formalin inflammatory pain was observed. Meanwhile, the foot thickness of mice before and60 min after establishment of formalin pain model was compared. Results All mice displayed apparent dual-phase pain response after subcutaneously injected formalin. In phase-1, there were no significant differences on mice licking/biting foot times among low-dose group [（88.88±6.98） s], high dose group [（90.09±8.49） s] and vehicle group [（90.50±11.18） s]（P〈0.05）. In phase-2, compared with vehicle group [（220.25 ±80.80） s], mice licking/biting foot times of low-dose group [（81.25±14.10） s] and high-dose group [（50.63±10.77） s] were significantly shorten, with statistical differences（P〈0.01）. Compared with vehicle group [（1.78±0.02） mm], plantar thickness of mice in low-dose group [（1.31±0.07） mm]and high-dose group [（1.11±0.08） mm] were significantly reduced, with statistical differences（P〈0.01）. Conclusion Caspase-6 inhibitor Z-VEID-FMK can relieve formalin inflammatory pain and may be related to its anti-inflammatory effect.

关 键 词：CASPASE-6 Z-VEID-FMK 福尔马林炎性痛 足底厚度 

分 类 号：Q332[生物学—遗传学]