恩替卡韦和干扰素序贯作用对HepG2.2.15细胞HBV DNA复制的影响  

The effect on replication of HBV DNA in HepG2.2.15 cells by the sequential treatment with Entecavir following interferon α-2b

作　　者：杨伟[1] 赵超[1] 李婧[1] 丁扬[1] 王雪[1] 

机构地区：[1]解放军第89医院传染科,山东潍坊261021

出　　处：《胃肠病学和肝病学杂志》2016年第4期408-410,共3页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的探讨恩替卡韦(Entecavir,ETV)与干扰素(IFNα-2b)序贯作用对Hep G2.2.15细胞HBV DNA复制的影响。方法将培养Hep G2.2.15细胞分组,单独组中分别加入不同浓度ETV的配制液(1.5μg/ml、5μg/ml、10μg/ml)或IFNα-2b配制液(5 000 IU/ml、10 000 IU/ml、15 000 IU/ml);联合组中联合加入ETV和IFNα-2b配制液;序贯组中先后加入ETV和IFNα-2b序贯配制液。在不同时点,采用MTT法检测Hep G2.2.15细胞增殖情况,采用实时荧光定量法检测各组细胞培养上清液中HBV DNA水平,酶联免疫法(ELISA)检测上清液中HBs Ag与HBe Ag水平。结果随着时间延长,序贯组对Hep G2.2.15的HBV DNA复制抑制作用明显大于其他两组(P<0.05);对HBs Ag与HBe Ag的抑制作用随时间的延长而加强(P<0.05)。结论 ETV与IFNα-2b序贯作用于Hep G2.2.15细胞,对Hep G2.2.15细胞HBV DNA复制有明显的抑制作用,且作用强于单独或联合使用。Objective To investigate the effect on replication of HBV DAN in Hep G2. 2. 15 cells by the sequential treatment with Entecavir（ ETV） following interferon α-2b（ IFNα-2b）. Methods In individual groups,Hep G2. 2. 15 cells were treated with various concentrations of ETV（1. 5 μg / ml,5 μg / ml,10 μg / ml） and IFNα-2b（5 000 IU / ml,10 000 IU / ml,15 000 IU / ml） individually; in combination group,Hep G2. 2. 15 cells were treated with ETV and IFNα-2b combination; in sequential groups,Hep G 2. 2. 15 cells were treated with ETV following IFNα-2b. The method of determined proliferation was MTT; HBV DNA copies were detected by Real time PCR-fluorescence probing in onetube method from the supermatant of Hep G 2. 2. 15 cells; HBs Ag and HBe Ag expressions from the supermatant were measured by ELISA at different time points. Results Compared with the individual groups or the combination group,sequential group had more powerful inhibition effects on HBV DNA replication,the more powerful inhibition expressions of HBs Ag and HBe Ag in Hep G2. 2. 15 cells. Conclusion The sequential treatment with ETV following IFNα-2b has more powerful effect on replication of HBV DNA than the individual treatment or combination treatment.

关 键 词：恩替卡韦 干扰素Α-2B HEPG2.2.15细胞 HBV DNA 序贯作用 

分 类 号：R512.62[医药卫生—内科学]