低氮胁迫下香蕉硝酸盐转运蛋白MaNRT2基因的克隆与表达分析  

Cloning and Expression Analysis of Nitrate Transporter Gene MaNRT2 in Banana under Low Nitrogen Stress

作　　者：贺瑞[1] 彭赟[1] 刘扬[1] 曾灿彬 徐敏[1] 齐钊[1] 闫臻[1] 骆娟[1] 阮云泽[1] 何朝族[1] 汤华[1] 

机构地区：[1]海南大学海南省热带生物资源可持续利用重点实验室/农学院,海口570228

出　　处：《热带生物学报》2016年第1期76-81,共6页Journal of Tropical Biology

基　　金：国家自然科学基金资助项目(31360364);教育部热带作物新品种选育工程中心与作物学重点学科联合资助项目(lhxm-2012-2);海南省自然科学基金(311025);海南省重大科技项目(ZDZX2013023);"中央财政支持中西部高校提升综合实力专项"香蕉子课题

摘　　要：氮素是植物生长发育所必需的大量元素,也是限制植物产量的首要因素,硝酸盐转运蛋白NRT是植物吸收和利用氮素的重要蛋白。笔者以香蕉为实验材料,通过RNA-Seq测序,筛选得到一个显著差异表达的高亲和硝酸盐转运蛋白基因NRT2;通过PCR克隆获得1 509 bp的c DNA序列,生物信息学预测其编码502氨基酸,含有MFS结构域,属于NRT2基因家族,命名为MaNRT2;RNA-Seq和qRT-PCR的结果显示,MaNRT2基因的表达具有显著的组织特异性,在根中远高于叶片;低氮胁迫处理后,MaNRT2在叶片中表达量上升,在根中表达量反而下降,表明MaNRT2与香蕉氮元素的吸收转运密切相关。Nitrogen is a necessary macro-element for plant growth and development,and is also a key factor limiting plant yield. High affinity nitrate transporter NRT2 is an important protein involved in nitrate nitrogen assimilation. In this study,banana was selected as experimental material,from which a differentially expressed NRT2 gene was identified by Illumina RNA-Seq. The whole c DNA sequence was obtained via PCR. Through bioinformatics,it was predicted that its length was 1 509 bp which encoded 503 amino acids,contained a MFS domain and belonged to NRT2 gene family named as MaNRT2. The results of RNA-Seq and qRT-PCR showed that MaNRT2 was significantly tissue specific,with its expression being higher in root than in leaf. After the banana was treated with low nitrogen stress,the MaNRT2 expression level rose up in leaf but declined in root. This indicated that MaNRT2 gene had close relationship to nitrogen absorption and transport in banana.

关 键 词：香蕉 低氮胁迫 硝酸盐转运蛋白2 转录组测序 基因表达分析 

分 类 号：S668.1[农业科学—果树学]