内生菌对柳树生长及氮磷吸收的强化作用  

Bioaugmentation effects of endophytic bacteria on growth and nitrogen and phosphorus accumulation of willow

作　　者：刘桂青[1] 杨栋[2] 倪其军[2] 李廷强[1] 郝虎林 陈宝[1] 杨卫东[1] 杨肖娥[1] 

机构地区：[1]浙江大学环境与资源学院,污染环境修复与生态健康教育部重点实验室,杭州310058 [2]中国船舶重工集团公司第七○二研究所,江苏无锡214082 [3]宁波原水研究院,浙江宁波315100

出　　处：《浙江大学学报（农业与生命科学版）》2016年第2期256-264,共9页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家科技支撑计划(2012BAC17B03-02);国际合作专项与支撑项目(2010DFB33960,2012BAC17B00);浙江省宁波市科技局重点项目(2012C10003);浙江省水利厅重点项目(RB1310);中央高校科研发展专项(2015FZA6008)

摘　　要：采用水培试验,比较了8株来源于超积累植物的内生菌(SaMR12、SaZR4、SaMR10、SaNR1、SaCS20、SM03、SM05和LM02)对柳树生长及氮磷吸收的影响。结果表明:接种SaMR12和SaMR10后柳树叶绿素含量显著增加,分别比对照提高了15%和14%;接种LM02、SaNR1和SaMR12对柳树根系生长有明显的促进作用,其中柳树的总根长比对照分别增加了163%、136%和66%,根表面积分别增加了54%、12%和17%,根尖数分别增加了54%、44%和48%;接种LM02、SaMR12和SaMR10后柳树总生物量分别比对照增加了234%、43%和54%;LM02、SaMR12和SaMR10这3株内生菌能显著促进柳树氮磷吸收,其中柳树地上部氮积累量比对照分别增加了176%、26%和41%,根系氮积累量分别是对照的8.3倍、2.4倍和3.4倍,柳树地上部磷积累量比对照分别增加了109%、12%和30%,根系磷积累量分别是对照的4.8倍、2.1倍和2.8倍。研究结果显示,接种LM02、SaMR10和SaMR12对柳树生长和氮磷积累有显著的促进作用,同时,显著提高了对富营养化水体的修复效率。Due to large biomass,submergence tolerance,rapid growth,well-developed root system and strong adaptability,willow（Salix matsudana）trees were not only widely used in ecological restoration of the heavy metal pollution,organic pollution and eutrophication,but also used as source of biomass energy.To expand the range of application and improve the economic value,it is necessary to enhance the biomass and nutrient accumulation of willow.Phyto-microbial remediation technology was widely used in recent years for it wasenvironmentally friendly,low cost and high efficiency in restoration.With assistance of microbe,the efficiency of phytoremediation was significantly enhanced.Endophytic bacterium,a kind of microbe colonized in plant tissue without causing pathogenicity,could produce plant hormones like gibberellin and indole acetic acid（IAA）to promote plant growth.In this study,willow trees were inoculated by eight endophytic bacteria（SaMR12,SaZR4,SaMR10,SaNR1,SaCS20,SM03,SM05 and LM02）derived from hyperaccumulator Sedum alfredii,and the plant growth and accumulation of nitrogen and phosphorus were investigated.In hydroponics experiment,the willow cuttings were grown in 2.5 L Hoagland＇s solution with physiology upward until root length was above 1cm.And propagation of endophytic bacteria suspension was added to the vessel with final density of 1×108 CFU/mL.The bacterial solution was replaced after 3days.The treatment without bacterial inoculation was used as a control.The willows were observed and determined after 2months of culture.The chlorophyll contents,root morphology,shoot and root biomass,nitrogen and phosphorus accumulation showed significantly different among different endophytic bacteria treatments. Chlorophyll content was significantly increased by 15% and 14% with inoculation of SaMR12 and SaMR10endophytic strains as compared with the control.After inoculation with LM02,SaNR1 and SaMR12,the root morphology was significantly improved.The total root length was increased by 163%,136% and 66%,respec

关 键 词：内生细菌 柳树 生物量 氮 磷 生物强化 

分 类 号：S792.12[农业科学—林木遗传育种] Q89[农业科学—林学]