模拟氮沉降对三江平原小叶章湿地土壤细菌多样性的影响  

Effect of simulation nitrogen depositions on bacterial diversity of Deyeuxia angustifoliain wetland of Sanjiang Plain

作　　者：隋心[1,2] 张荣涛[2] 杨立宾[2] 许楠[2] 柴春荣[2] 王继丰[2] 付晓玲[2] 钟海秀[2] 邢军会[2] 张玉[2] 倪红伟[2] 

机构地区：[1]东北林业大学林学院,黑龙江哈尔滨150040 [2]黑龙江省科学院自然与生态研究所湿地与生态保育国家地方联合工程实验室,黑龙江哈尔滨150040

出　　处：《草业科学》2016年第4期589-598,共10页Pratacultural Science

基　　金：国家自然科学基金项目(31470019);黑龙江省科研机构创新能力提升专项计划(GY2014KC0039);黑龙江省科学院院级项目(STJB16-01;STJB16-04;2015YZ01;2014ST05)

摘　　要：利用黑龙江省科学院自然与生态研究所三江平原湿地生态定位研究站内的模拟氮沉降试验平台,应用Miseq测序技术对细菌16SrDNA进行序列测定和分析,探讨添加氮对三江平原小叶章湿地土壤细菌群落结构的影响。结果表明,与对照相比,添加氮增加土壤细菌群落多样性,低氮(4g·hm^(-2)·a^(-1))时土壤细菌多样性最高,高氮(8g·hm^(-2)·a^(-1))次之。模拟氮沉降导致湿地土壤细菌群落的结构以及丰度发生变化,低氮、高氮和对照地的优势种群均为酸杆菌门(Acidobacteria),变形菌门(Proteobacteria)次之,随着氮沉降的增加,酸杆菌门的丰度逐渐下降,变形菌门的丰度则先升高后降低。外源氮的输入使得土壤的理化性质发生了改变,导致细菌群落的组成以及细菌的相对丰度发生改变。16SrDNA heatmap分析则表明,不同的细菌群落对氮沉降的响应不同。本研究为预测未来大气氮沉降对三江平原湿地土壤微生物的影响以及湿地生态系统的变化提供基础数据和理论参考。Wetland soil bacterial community structure under the simulation nitrogen depositions was studied using 16 SrDNA gene sequencing.The soil samples were collected from three different nitrogen depositions in the Sanjiang Plain Field Experiment Station of the Institute of Nature Ecology,Heilongjiang Academy Sciences.Total microbial DNA was directly extracted from soil samples and 16 SrDNA genes were sequenced and analyzed by Miseq molecular technology.The results showed that both low N and high N treatment increased the bacterial diversity compared with control treatment and low N treatment had the highest diversity.The wetland bacterial community structures significantly changed under simulation N depositions.In low N treatment,high N treatment and control treatment,Acidobacteria were predominated followed by Proteobacteria.The richness of Acidobacteria decreased and the richness of Proteobacteria increased first then decreased along the deposition.Soil pH decreased with deposition,which showing acidic property and increasing the Acidobacteria distribution.Soil physicochemical properties changed by input N which resulted in the soil bacterial community composition and abundant changed.The heatmap tree based on the 16 SrDNA sequences showed that different bacterial community structure differently responded on Nitrogen deposition.This research provided basic data and theory consultation for predicting the influence of future atmospheric nitrogen deposition on soil microorganism in Sanjiang Plain wetlands.

关 键 词：16SrDNA 小叶章湿地 Miseq 氮沉降 细菌多样性 

分 类 号：S154.36[农业科学—土壤学]