结核分枝杆菌对巨噬细胞岩藻糖转移酶家族基因表达的影响  

Influence of Mycobacterium tuberculosis stimulation on the expression of fucosyltransferases in macrophages

作　　者：胡锋 潘勤[1] 章晓联[1] 

机构地区：[1]武汉大学病毒学国家重点实验室/湖北省过敏及免疫相关疾病重点实验室/武汉大学医学研究院/武汉大学基础医学院免疫学系,湖北武汉430071

出　　处：《中国生化药物杂志》2016年第3期19-22,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家重大传染病专项(2012ZX10003002-015);国家自然科学基金项目(31221061;31270176;81471910;31370197;21572173);湖北省医学领军人才项目(523-276003)

摘　　要：目的研究结核分枝杆菌标准株H37Rv(inactivated H37Rv,i H37Rv)刺激巨噬细胞RAW264.7细胞后,对岩藻糖转移酶家族中的10个糖基转移酶表达的影响。方法用热灭活的i H37Rv刺激小鼠巨噬细胞系RAW264.7,用qRT-PCR(quantitative realtime polymerase chain reaction)检测i H37Rv分别刺激RAW264.7细胞0 h、4 h、8 h、12 h后对巨噬细胞中10个岩藻糖基转移酶的mRNA表达水平的影响。凝集素染色和流式细胞术检测岩藻糖转移酶对应的糖苷的表达变化。结果 i H37Rv分别刺激RAW264.7细胞0 h、4 h、8 h、12 h后,岩藻糖转移酶1(FUT1)基因的表达随着刺激时间的延长而逐步下调。凝集素染色和流式细胞术检测显示特异性结合岩藻糖转移酶-FUT1对应糖苷的小扁豆凝集素(lens culinaris agglutinin,LCA)在i H37Rv刺激巨噬细胞12 h后结合巨噬细胞上对应的糖苷的能力下降了4.6%。结论 FUT1糖基转移酶在i H37Rv刺激巨噬细胞后发生下调,其有可能作为结核分枝杆菌感染的诊断分子标志和治疗新靶点。Objective To study after Mycobacterium tuberculosis（ MTB） H37 Rv stimulation on mouse macrophage RAW 264. 7 cell lines,the effect on the expression of Fucose transfer 10 glycosyl transferase enzyme family. Methods RAW264. 7 cell lines were treated with heat inactivated H37Rv（ i H37Rv） stimulation for 0 h,4 h,8 h and 12 h,qRT-PCR was used to measure the expressions of mRNA of fucosyltransferases. Lectin staining and flow cytometry were used to examine the binding ability of lectin Lens culinaris agglutinin（ LCA） to glycoprotein ligand by fucosyltransferase on macrophages. Results The expression of FUT1 were found progressively decreased in RAW 264. 7 cells at different time points of i H37 Rv stimulation and the macrophage binding ability of lectin LCA which specifically binds to glycoprotein ligand by fucosyltransferase decreased 4. 6% after 12 h of i H37 Rv stimulation. Conclusion The expression of FUT1 in macrophages decrease after i H37 Rv stimulation and thus FUT1 has potentials as a diagnosis molecular marker or drug target for tuberculosis.

关 键 词：结核分枝杆菌 巨噬细胞 糖基转移酶 

分 类 号：S852.61[农业科学—基础兽医学]