云南榧种子内生真菌的分离鉴定及抑菌活性筛选  

Isolation and Identification of Endophytes from Seeds of Torreya yunnanensis and Its Antimicrobial Activity Screening

作　　者：李向楠[1] 周稚凡[1] 刘风路[1] 李靖[1] 

机构地区：[1]西南林业大学生命科学学院,云南昆明650224

出　　处：《生物灾害科学》2016年第1期20-26,共7页Biological Disaster Science

基　　金：云南省生物学优势特色重点学科建设项目(50097505)

摘　　要：对采自云南省维西县的云南榧(Torreya yunnanensis)种子的内生真菌进行分离纯化和初步形态学鉴定,同时对内生真菌的抑菌活性进行筛选。通过真菌分离纯化技术对云南榧种子的内生真菌进行分离纯化,通过显微镜观察产孢结构进行初步形态学分析,并通过滤纸片法对内生真菌的抑菌活性进行筛选。从云南榧种子中共分离纯化得到50株内生真菌,其中拟茎点霉属13株,镰孢霉属7株,炭疽霉属5株,拟青霉属4株,链格孢属1株,木霉属1株,无孢菌群19株。抑菌活性显示云南榧种子内生真菌具有广泛的抑菌活性,其中菌株Z41与Z43对10种植物病原真菌具有广谱抗性。In this study, the endophytic fungi were isolated and purified from the seeds of T.yunnanensis collected from Weixi County, Yunnan Province. The morphology of the endophytic fungi was identified,and antimicrobial activity was screened. By fungal separation and purification technology, endophytic fungi were isolated initially from the seeds of T.yunnanensis. Morphological analyses of endophytic fungi were conducted by microscopic observation of sporulation structures, and antibacterial activities were screened by filter paper method. Fifty endophytic fungi were isolated and purified. Among them, 13 belonged to Phomopsis, 7 Fusarium, 5 Anthrax, 4 Paecilomyces, 1 Alternaria, 1 Trichoderma, and 19 without spores. Antimicrobial results showed that crude extracts of endophytic fungi had broader inhibitory effects on ten important plant pathogenic fungi. Z41 and Z43 exhibited the best antimicrobial activities.

关 键 词：云南榧 内生菌 分离纯化 活性筛选 滤纸片法 

分 类 号：Q949.32[生物学—植物学]