益气补肾活血方对大鼠退变腰椎间盘中OP-1及IL-1的影响  

Effects of Supplementing Qi and Nourishing Kidney and Promoting Blood Circulation Decoction(益气补肾活血方) on OP-1 and IL-1 in Rats with Lumbar Intervertebral Disc Degeneration

作　　者：林斌 潘永亮 蔡金亮[3] 毕宗伟[3] 章瑞峰 李俊杰[4] 钟道柱 

机构地区：[1]69064部队门诊部,新疆库尔勒841000 [2]解放军第二七三医院,新疆库尔勒841000 [3]69064部队卫生处,新疆库尔勒841000 [4]湖南中医药大学,湖南长沙410208

出　　处：《中医药导报》2016年第8期20-22,共3页Guiding Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:探讨益气补肾活血方对大鼠退变腰椎间盘中OP-1及IL-1的影响。方法:将60只大鼠随机分为对照组、实验组、假手术组,每组20只;采用腰椎间盘纤维环穿刺法建立腰椎间盘退变模型,术后第1天,实验组予益气补肾活血中药浓缩液灌胃,术后第4、8周,每组各取5只大鼠,HE染色观察椎间盘组织形态学改变,ELISA法观察椎间盘中OP-1、IL-1含量。结果:HE染色结果可见实验组大鼠椎间盘退变程度低于对照组;实验组及对照组大鼠造模后4、8周椎间盘中OP-1含量均低于假手术组(P<0.05),IL-1含量均高于假手术组(P<0.05);实验组大鼠腰椎间盘中OP-1含量高于对照组(P<0.05),IL-1含量低于对照组(P<0.05)。结论:益气补肾活血方可调节大鼠退变腰椎间盘中OP-1及IL-1的含量,减缓椎间盘退变。Objective： To investigate the effects of supplementing qi and nourishing kidney and promoting blood circulation decoction （益气补肾活血方 SQNKPBCD） on OP-1 and IL-1 in Rats with lumbar intervertebral disc degeneration. Methods： The 60 rats were randomly divided into control group, the experimental group and sham group, and each group had 20 cases. Establish lumbar disc degeneration model from outside of the abdomen. After one day of the operation, the experimental group began administering traditional Chinese medicine and concentrates lavage. After 4 weeks and 8 weeks of the operation, 5 rats were selected in each group, the intervertebral disc tissue morphological changes were observed, and the intervertebral disc degeneration in the OP-1 and IL-1 content of the remaining five specimens in each group were detected. Results： HE staining results showed that the intervertebral disc degeneration in the experimental group was lower than that in the control group. The contents of OP-1 in experimental group and control group were lower than that in sham group （P〈0.05）, and the Cortents of IL-1 in experimental gronp and control gronp were hgher than that in sham group （P〈0.05）. The contents of OP-1 in experimental group were higher than the control group （P〈0.05）, while the IL-1 was lower than control group. Conclusion： SQNKPBCD can regulate the content of OP-1 and IL-1 in degenerated lumbar discs, and slow down the degeneration.

关 键 词：益气补肾活血方 腰椎间盘退行性改变 OP-1 IL-1 大鼠 

分 类 号：R285.5[医药卫生—中药学]