二维及微团培养法对软骨细胞表型的影响  

Effects of 2D culture and micropellet culture on the chondrocyte phenotype

作　　者：宋雄波 吴江怡 黄术[1] 陈诚[3] 杨柳[3] 刘宝荣[1] 

机构地区：[1]湖南省人民医院/湖南师范大学第一附属医院关节外科,长沙410005 [2]南华大学第一附属医院骨科,湖南衡阳421001 [3]第三军医大学西南医院关节外科,重庆400038

出　　处：《重庆医学》2016年第11期1456-1458,1462,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(31300773);中国博士后科学基金资助项目(2013M532152)

摘　　要：目的探讨二维及微团培养对软骨细胞表型的影响。方法体外高密度二维及微团培养方法培养兔软骨细胞,从细胞形态、细胞外基质表达情况分析软骨细胞表型的差异。结果高密度二维方案培养兔软骨细胞,2周后软骨细胞多层分布,HE染色显示细胞呈圆形,细胞间联系较密,阿尔新蓝染色显示细胞外蛋白多糖分泌较少;微团培养方法培养兔软骨细胞,2周后形成肉眼可见的白色组织,HE染色显示细胞呈多边形,细胞较分散,阿尔新蓝染色显示细胞外蛋白多糖分泌明显多于二维培养,差异有统计学意义(P<0.05)。结论微团培养方法培养兔软骨细胞,有利于软骨细胞分泌细胞外基质。Objective To analyze the effects of 2D culture and micropellet culture on the chondrocyte phenotype.Methods High density 2D culture and micropellet culture were used to culture rabbit chondrocytes,the cell morphology and level of matrix expression were measured.Results After 2 weeks of 2D high density culture,multilayer chondrocyte distribution was observed.HE staining showed that the cells were round in shape,and were closely connected with each other,alcian blue staining showed that the secretion of proteoglycan was low;after 2 weeks of micropellet culture,cartilage-like white tissue was formed.HE staining showed that the cells were polygonal in shape,and were sparsely distributed,alcian blue staining showed that the secretion of proteoglycan was significantly higher than that in 2D culture（P〈0.05）.Conclusion Using micropellet to culture rabbit chondrocytes is favorable for the secretion of extracellular matrix.

关 键 词：软骨细胞 二维培养 微团培养 软骨表型 

分 类 号：R684[医药卫生—骨科学]