多孔块体生物活性玻璃在兔体内成骨活性、生物力学性能的初步研究  

Influence of macro-pore bone block on osteogenic activity and mechanical property in vivo

作　　者：胡腾龙[1] 厉晓杰 张斌[2] 杨柳[1] 颉强[1] 

机构地区：[1]第四军医大学西京医院骨科,西安710032 [2]第四军医大学西京医院急诊科,西安710032

出　　处：《中华骨科杂志》2016年第8期479-489,共11页Chinese Journal of Orthopaedics

基　　金：国家自然科学基金面上项目（81472043）

摘　　要：目的探讨多孔块体生物活性玻璃植入兔体内后的生物活性、生物相容性、成骨能力以及生物力学性能。方法制备新西兰大白兔双侧股骨髁骨缺损模型，分别于股骨髁骨缺损部植入多孔块体生物活性玻璃、β-磷酸三钙、固骼生（NOVABONE），按植入材料不同分为多孔块体生物活性玻璃组、β-磷酸三钙组和固骼生组。术后摄X线片观察材料置入情况及固定是否牢固、髁部有无骨折。分别于术后4、12和24周取材，通过Micro—CT检测植入材料的新骨生成体积百分比及剩余材料体积百分比；采用四环素、钙黄绿素荧光双标检测三组材料的新骨生成速率；通过不脱钙硬组织VanGieson染色检测三组材料的新骨生成面积百分比；通过生物力学测试三组材料的压缩强度、弹性模量。结果术后X线检查显示各组材料填充充分、完全，置入情况良好。Mocro—CT结果显示，24周时多孔块体生物活性玻璃组、β-磷酸三钙组、固骼生组的新骨生成百分比分别为（37．48％±0．70％，25．29％±1．45％，27．03％±1．25％），多孔块体生物活性玻璃组与β-磷酸三钙组、固骼生组比较差异均具有统计学意义，各组材料剩余体积百分比分别为（34．67％±3．52％，55．66％±2．05％，7．52％±1．15％），多孔块体生物活性玻璃组与β-磷酸三钙组、固骼生组比较差异均有统计学意义。荧光双标结果显示，4周时多孔块体生物活性玻璃组、β-磷酸三钙组和固骼生组的新骨生成速率分别为（1．577±0．045）μm／d、（2．064±0．068）μm／d和（1．19_0．09）μm／d，多孔块体生物活性玻璃组与β-磷酸三钙组的差异有统计学意义；VanGieson染色检测显示，多孔块体生物活性玻璃组、β-磷酸三钙组和固骼生组新骨生成面积百分比分别为4周时（5.43％±1．25％、2．77％±0．85％和6．51％±1．21％），12周时（8．48％Objective To observe and identify the osteogenic activity, biocompatibility and mechanical property of a type of macro-pore bone block bioactive glass in rabbits. Methods Establish the femoral condyle defect model with New Zealand white rabbit. Implant in the defect with macro-pore bone block bioglass, β-TCP and NOVABONE respectively. According to the different materials implanted in the defect, three groups were divided as macro-pore bone block bioglass group, β-TCP group and NOVABONE group. After the surgery, X-ray examination was performed to confirm the location and fixation of the materials and to observe the femoral condyle fracture. The specimens were harvested at 4, 12 and 24 weeks after the surgery respectively. Micro- CT was performed to assess the new bone formation and degradation of the materials. Tetracycline-calcein double labeling was used to detect the mineral apposition rate of new bone. Van Gieson staining was used to assess the new bone formation percentage. Biomechanical markers including the compress strength and elasticity modulus were also measured. Results X-ray examination showed that each femoral defect was filled fully with materials and the materials were all in proper position. As indicated by Micro- CT results, at 24 weeks, the bone regeneration volume fraction of each group was 37.48%±0.70%, 25.29%± 1.45%, 27.03%± 1.25% respectively and the difference between macro-pore bone block group and β-TCP group or NOVABONE group was statistically significant. The residual material volume fraction of each group was 34.67%±3.52%, 55.66%±2.05%, 7.52± 1.15% respectively and the difference between macro-pore bone block group and β-TCP group or NOVABONE group was statistically significant. The results of tetracycline-calcein double labeling showed that the mineral apposition rate in macro-pore bone block bioglass group, β-TCP group and NOVABONE group at 4 weeks was （1.577±0.045） μm/d, （2.064±0.068）μm/d, （1.19±0.09）μm/d respectively and the difference between macro-por

关 键 词：骨代用品 硅酸盐类 材料试验 骨生成 生物力学 

分 类 号：R318.08[医药卫生—生物医学工程]