白蜡虫蜡酯合酶在昆虫细胞Sf9中的表达  

Expression of Ericerus pela Wax Ester Synthase in Spodoptera frugiperda Sf9 Cell Line

作　　者：亓倩[1] 于淑惠[1] 孙涛[1] 王雪庆 刘博文[1] 杨璞[1] 陈晓鸣[1] 

机构地区：[1]中国林业科学研究院资源昆虫研究所,国家林业局资源昆虫培育与利用重点实验室,云南昆明650224

出　　处：《林业科学研究》2016年第2期191-195,共5页Forest Research

基　　金：林业公益性行业科研专项201504302,201304808;云南省应用基础研究重点项目2013FA052;国家自然科学基金31572337;国家863计划2014AA021801

摘　　要：[目的]本研究欲利用Bac-to-Bac表达系统,在Sf9细胞中表达WS。[方法]将WS编码序列克隆到质粒p FastBac^(TM)HT B后,转化大肠杆菌DH10Bac^(TM),经抗性和蓝白斑筛选,获得重组杆粒r Bacmid/Epel WS。将r Bacmid/EpelWS转染Sf9细胞。[结果]免疫荧光标记检测表明:WS在受感染细胞的细胞质中表达。[结论]本研究成功表达了白蜡虫WS,为白蜡虫WS的生化特性研究奠定了基础。[Objective]Use Bac-to-Bac expression system to express WS in Sf9 cell line. [Method]The WS coding sequence was cloned into plasmid p Fast Bac^（TM）HT B,and then transformed Escherichia coli DH10 Bac^（TM）. After resistant and blue-white screening,the recombinant bacmid r Bacmid / Epel WS were obtained. The r Bacmid / Epel WS was transfected into Sf9 cells. [Result]The Immunofluorescence analysis showed that the target protein was expressed in the cytoplasm of the infected cells. [Conclusion]The WS was expressed successfully,it lays the foundation for further research on the biochemical characteristics of WS of the Ericerus pela.

关 键 词：白蜡虫 蜡酯合酶 载体构建 Bac-to-Bac表达系统 Sf9细胞系 

分 类 号：S899.1[农业科学—特种经济动物饲养]