IgA肾病患者外周血单核细胞含量及肾组织中NF-κB、CTGF、OPN表达量的检测及临床意义  

Detection of peripheral blood mononuclear cell content as well as NF-κB,CTGF and OPN expression in kidney tissue of patients with IgA nephropathy and its clinical significance

作　　者：郝成罗[1] 廖常彬[1] 

机构地区：[1]自贡市第三人民医院肾病内科

出　　处：《海南医学院学报》2016年第11期1079-1081,1085,共4页Journal of Hainan Medical University

基　　金：自贡市重点科研项目(2013zc27)~~

摘　　要：目的：研究IgA肾病患者外周血单核细胞含量及肾组织中NF-κB、CTGF、OPN表达量及临床意义。方法：选择在本院确诊为IgA肾病的25例肾病患者为肾病组,因外伤或肿瘤接受肾脏切除的25例患者为对照组进行研究,采集外周血和肾脏组织,测定NF-κB、CTGF、OPN、T-bet、GATA-3、RORγt、Foxp3表达量。结果：肾病组外周血单核细胞和肾脏组织中CTGF、OPN的百分比高于对照组;M1、E1、S1组外周血单核细胞和肾组织中NF-κB、CTGF、OPN的表达量明显高于M0、E1、S1组;肾病组外周血中T-bet、GATA-3、RORγt的表达量明显高于对照组且与NF-κB、CTGF、OPN的表达量呈正相关;Foxp3的表达量明显低于对照组且与NF-κB、CTGF、OPN的表达量呈负相关。结论：IgA肾病患者外周血单核细胞含量及肾组织中NF-κB、CTGF、OPN的表达量异常升高且能够评估疾病的预后情况以及CD4^＋T细胞的分化情况。Objective：To study the expression and clinical significance of NF-κB,CTGF and OPN in mononuclear cells in peripheral blood as well as renal tissues in patients with IgA nephropathy.Methods：A total of 25 nephropathy patients diagnosed with IgA nephropathy and 25 cases of control patients receiving nephrectomy due to trauma or tumor in our hospital were studied.Peripheral blood and kidney tissues were collected to test NF-κB,CTGF,OPN,T-bet,GATA-3,RORγT and Foxp3 expressions.Results：CTGF and OPN percentages in peripheral blood mononuclear cells and kidney tissues of nephropathy patients were higher than those of the control group.NF-κB,CTGF and OPN expressions were significantly higher in M1,E1,S1 group patients＇ peripheral blood mononuclear cells and renal tissues than those of M0,E1 and S1 group＇s.T-bet,GATA-3 and RORγT expressions in nephropathy patients＇ peripheral blood were significantly higher than those in the control group and were positively correlated with NF-κB,CTGF and OPN expressions levels.The expression of Foxp3 was significantly lower than that of control group,and was negatively correlated with NF-κB,CTGF and OPN expressions.Conclusions：The expression of NF-κB,CTGF and OPN in peripheral blood mononuclear cells and renal tissue in patients with IgA nephropathy is abnormally high and can evaluate the prognosis of the disease and the differentiation of CD4^＋ T cells.

关 键 词：IGA肾病 NF-ΚB 结缔组织生长因子 骨桥蛋白 CD4+T细胞 

分 类 号：R692[医药卫生—泌尿科学]