日光灯光照对替加环素活性及体外药敏试验结果的影响  

Study on the impact of fl uorescent light in inactivating tigecycline and performing susceptibility testing in vivo

作　　者：陈华乐[1] 李梅梅[1] 李斌[1] 侯渊博 周铁丽[1] 

机构地区：[1]温州医科大学附属第一医院医学检验中心,温州325035 [2]温州医科大学检验医学院生命科学学院,温州325035

出　　处：《中国抗生素杂志》2016年第4期301-304,共4页Chinese Journal of Antibiotics

基　　金：国家自然科学基金(No.81171614);浙江省卫生高层次创新人才培养工程项目(浙卫发[2012]241号)

摘　　要：目的探讨日光灯光照对替加环素抗菌活性的影响,为确定替加环素体外药敏试验合适的照明条件及药物临床使用的稳定性提供依据。方法采用微量肉汤稀释法检测不同光照时间处理后的替加环素药液对大肠埃希菌和鲍曼不动杆菌最低抑菌浓度(MIC值),并采用高效液相色谱法(HPLC)检测替加环素有效成分的变化。结果体外药敏结果显示经光照处理的各组替加环素抗菌活性均未发生明显变化。HPLC结果显示经光照处理的各组替加环素与对照组相比,有效成分也都未发生明显改变。结论替加环素液体制剂形式在实验室和临床操作过程中无须严格避光,日光灯源可直接作为照明光源。Objective The investigate the impact of fluorescent light in inactivating the antimicrobial activity of tigecycline and suitable lighting condition for susceptibility testing in vivo and clinical using. Methods Broth microdilution method was performed to mensurate minimal inhibitory concentrations （MIC） of each treated liquid preparation of tigecycline. To further confirm the influence of fluorescent light, high performance liquid chromatography （HPLC） was employed to detect the concentration of tigecycline precisely. Results Value of susceptibility testing using treated tigecycline was consistent with known MICs value indicating no notable effect of fluorescent light on inactivating the antimicrobial activety of tigecycline. HPLC also showed no significant difference between treated group and control group. Conclusion The fluorescent light can be used to perform laboratory and clinical operation using liquid preparation oftigecycline.

关 键 词：替加环素 体外药敏试验 高效液相色谱 

分 类 号：R978.1[医药卫生—药品]