野薄荷组织培养脱病毒技术研究  

Study on Virus-free Technique of Mentha haplocalyx by Tissue Culture

作　　者：谢文申[1] 付晏昆[2] 周露[1] 张青华[3] 

机构地区：[1]云南农业大学香料研究所,云南昆明650051 [2]昆明学院 [3]云南农业大学园林园艺学院

出　　处：《现代农业科技》2016年第6期61-62,64,共3页Modern Agricultural Science and Technology

基　　金：云南省教育厅科学研究基金项目(2014y203)

摘　　要：[目的]采用热处理结合茎尖分生组织培养的方法,研究薄荷组培脱毒技术。[方法]以薄荷组培苗为试材,在高温热处理不同天数后剥取茎尖,进行薄荷花《病毒脱毒技术研究,同时采用RT-PCR法检测其脱毒效果。[结果]适合薄荷热处理时间为20～45 d,试管苗的成活率可达66.7%～90.0%;32～38益热处理35～45 d剥取0.3～0.5 mm的茎尖进行培养,脱毒率为100.0%。[结论]热处理结合茎尖培养是一种脱除薄荷病毒的有效途径。[Objective] The technique of virus-free in Mentha haplocalyx was studied by combing tissue of shoot-tip with heating treatment. [Method] Mentha haplocalyx in vitro was used as test material in this experiment to eliminate by combing tissue of shoot-tip with heat therapy,and detected by indirect RT-PCR method.[Result] The suitable time for heat treatment was 20-45 days, the survival rate of tissue culture seedling was up to 66.7%-90.0% ;0.3--0.5 mm shoot-tip with heat treatment by 32-38 T; for 35-45 days of Mentha haplocalyx were 100.0% free from CMV and TMV. [Conclusion] Combing tissue of shoot-tip with heating treatment is an efficient way of virus-free in Mentha haplocalyx.

关 键 词：薄荷 组织培养 热处理 脱毒 RT-PCR 

分 类 号：S567.235[农业科学—中草药栽培]