Characterization of 24 microsatellite markers in Primula chungensis(Primulaceae),a distylous-homostylous species,using MiSeq sequencing  

作　　者：Wei Zhou Haidong Li Zhikun Wu Spencer C.H.Barrett Dezhu Li Hong Wang 

机构地区：[1]Plant Germplasm and Genomics Center,Germplasm Bank of Wild Species,Kunming Institute of Botany,Chinese Academy of Sciences [2]Key Laboratory for Plant Diversity and Biogeography of East Asia,Kunming Institute of Botany,Chinese Academy of Sciences [3]Kunming College of Life Sciences,University of Chinese Academy of Sciences [4]Kunming Botanical Garden,Kunming Institute of Botany,Chinese Academy of Sciences [5]Department of Ecology and Evolutionary Biology,University of Toronto

出　　处：《植物分类与资源学报》2016年第2期110-113,共4页Plant Diversity

基　　金：funded by the National Key Basic Research Program of China(2014CB954100);the Key Research Program of the Chinese Academic of Sciences(KJZD-EW-L07);the National Natural Science Foundation of China(31200289,31570384);the Natural Science Foundation of Yunnan Province(2012FB182)

摘　　要：Primula chungensis is a species with considerable floral and mating-system variation,including distylous(outcrossing),homostylous(selfing) and mixed populations that contain both outcrossing and selfing forms.We isolated 24 microsatellite markers from P.chungensis using Illumina Mi Seq sequencing.Polymorphism and genetic diversity were then measured based on a sample of 24 individuals from a natural population in southern Tibet.All loci were polymorphic with the number of alleles per locus ranging from 2 to 4.The observed and expected heterozygosity ranged from 0 to 1 and 0.219 to 0.708,respectively.The microsatellite markers we have identified will serve as valuable tools for the investigation of the population genetic structure and phylogeography of P.chungensis and will inform models of the evolutionary history of mating systems in the species.Primula chungensis is a species with considerable floral and mating-system variation, including distylous （outcrossing）, homostylous （selling） and mixed populations that contain both outcrossing and selling fornls. We isolated 24 microsatellite markers from P. chungensis using Illumina MiSeq sequencing. Polymorphism and genetic diversity were then measured based on a sample of 24 individuals from a natural population in southern Tibet. All loci were polymorphic with the number of alleles per locus ranging from 2 to 4. The observed and expected heterozygosity ranged from 0 to 1 and 0.219 to 0.708, respectively. The microsatellite markers we have identified will serve as valuable tools for the investigation of the population genetic structure and phylogeography of P.chungensis and will inform models of the evolutionary history of mating systems in the species.

关 键 词：DISTYLY Homostyly Microsatellites POLYMORPHISM Primula chungensis 

分 类 号：Q943.2[生物学—植物学]