抗日本血吸虫可溶性虫卵抗原IgY的制备及初步应用  

Preparation of anti-SEA egg yolk immunoglobulin and its preliminary application

作　　者：吴凯[1] 徐明星[1] 杨燕[1] 周水茂[1] 雷家慧[2] 刘文琪[2] 

机构地区：[1]湖北省武汉市疾病预防控制中心血吸虫病与地方病防治所 [2]华中科技大学同济医学院病原生物学系

出　　处：《中国血吸虫病防治杂志》2016年第2期196-198,共3页Chinese Journal of Schistosomiasis Control

基　　金：2013-2014年度湖北省卫生厅血吸虫病防治科研项目(XF2012-31)

摘　　要：目的制备抗日本血吸虫可溶性虫卵抗原(Soluble egg antigen,SEA)鸡卵黄免疫球蛋白(Egg yolk immunoglobulin,Ig Y),探讨其应用于血吸虫病流行区查病的可行性。方法在血吸虫病流行区采集间接红细胞凝集试验(IHA)阳性者尿液,在非流行区采集健康人尿液。以SEA经皮下注射免疫莱杭母鸡获得anti-SEA Ig Y,以十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)测定其相对分子量;并以anti-SEA Ig Y作为捕捉抗体,采用双抗体夹心法ELISA检测IHA阳性者和健康人尿液中的血吸虫循环可溶性虫卵抗原(Circulating soluble egg antigen,CSEA)。结果成功制备并纯化antiSEA Ig Y。共检测IHA阳性者尿液48份,在尿液中检出CSEA阳性26例,检出率为54.17%;检测健康人尿液10份,均为阴性。结论基于Ig Y的免疫诊断技术可有效检出人尿液中CSEA,其作为一种便捷、无创伤的新方法可应用于血吸虫病查病工作中。Objective To prepare the egg yolk immunoglobulin（Ig Y）against soluble egg antigen（SEA）of Schistosoma japonicum,and explore its feasibility for schistosomiasis screening in endemic areas.Methods The urine samples of indirect haemagglutination test（IHA）positives from endemic areas and healthy people from non-endemic areas were collected.The AntiSEA Ig Y was obtained through immunization by subcutaneous injection of SEA into Laihang hen,and its molecular weight was determined by SDS polyacrylamide gel electrophoresis（SDS-PAGE）.The circulating soluble egg antigen（CSEA）in the urine of IHA positives and healthy people were detected by ELISA-double antibody sandwich method with the Anti-SEA Ig Y as the capture antibody.Results Anti-SEA Ig Y was prepared and purified successfully.Totally 48 urine samples of IHA positives were detected by ELISA and 26 of them were CSEA positives（54.17%）;10 urine samples of healthy people were detected,and all of them were negative.Conclusions The CSEA in human urine can be detected effectively by ELISA based on Ig Y.As a convenient and atraumatic method,it could be applied in schistosomiasis screening.

关 键 词：血吸虫病 循环抗原 卵黄免疫球蛋白 尿液 免疫诊断 

分 类 号：R532.21[医药卫生—内科学]