绞股蓝皂苷和银杏叶提取物混合物对2型糖尿病并NAFLD大鼠血瘦素、脂联素和肝组织丙二醛、超氧化物歧化酶的影响  

Effects of gypenosides and ginkgo biloba extract mixture on plasma leptin,adiponectin,hepatic tissue malondialdehyde and superoxide dismutase in rats with type 2 diabetes mellitus and non-alcoholic fatty liver disease

作　　者：赵琴[1,2] 李儒贵[1] 李金科[1] 李芳[1] 李刚[1] 谭华炳[1] 

机构地区：[1]湖北医药学院附属人民医院感染性疾病科肝病研究所,湖北十堰442000 [2]湖北省房县人民医院检验科,湖北房县442100

出　　处：《中国肝脏病杂志（电子版）》2016年第1期62-66,共5页Chinese Journal of Liver Diseases：Electronic Version

基　　金：2009年十堰市科技局科技攻关指导项目(2009zd009)

摘　　要：目的观察绞股蓝皂苷（gypenosides,GPS）和银杏叶提取物（ginkgo biloba extract,GBE）混合物对2型糖尿病（type 2 diabetes mellitus,T2DM）并非酒精性脂肪性肝病（non-alcoholic fatty liver disease,NAFLD）大鼠血浆瘦素（leptin,LP）、脂联素（adiponectin,APN）和肝组织甘油三酯（TG）沉积量、丙二醛（malondialdehyde,MDA）、超氧化物歧化酶（superoxide dismutase,SOD）的影响。方法 40只SPF级雄性SD大鼠（体质量为220~250 g）随机分为空白对照组（空白组,n=7）,T2DM并NAFLD模型组（n=33）。造模周期8周。将T2DM并NAFLD模型大鼠随机分为GPS治疗对照组[简称对照组,给予GPS 0.5 g/（kg·d）灌胃,n=10],GPS和GBE混合物治疗组[简称治疗组,给予GPS 0.5 g/（kg·d）联合GBE 0.1 g/（kg·d）,n=10],模型对照组（简称模型组,给予同等体积的纯净水灌胃,n=10）。继续给予高糖高脂饲料,自由饮水,治疗周期为6周,实验共计14周。检测各组LP、APN、肝组织TG、MDA、SOD水平。结果①肝组织MDA变化：空白组（3.01±0.10）nmol/ml、模型组（4.78±0.12）nmol/ml、治疗组（3.60±0.09）nmol/ml、对照组（4.29±0.11）nmol/ml,治疗组低于对照组（t=15.35,P=0.000）。②肝组织SOD变化：空白组（268.4±15.2）U/ml、模型组（140.6±16.8）U/ml、治疗组（224.8±15.4）U/ml、对照组（180.6±16.0）U/ml,治疗组低于对照组（t=6.29,P=0.000）。③血APN变化：空白组（7.98±0.96）ng/ml、模型组（4.01±0.88）ng/ml、治疗组（5.92±0.12）ng/ml、对照组（5.11±0.10）ng/ml,治疗组低于对照组（t=16.40,P=0.000）。④血LP变化：空白组（2.82±0.58）ng/ml、模型组（7.89±0.68）ng/ml、治疗组（4.68±0.86）ng/ml、对照组（5.89±0.76）ng/ml,治疗组低于对照组（t=3.73,P=0.004）。⑤肝组织TG：空白组（30.26±2.48）mg/g、模型组（228.46±8.48）mg/g、治疗组（153.12±9.98）mg/g、对照组（196.24±9.78）mg/g,治疗组低于对照组（t=9.76,P=0.000）。�Objective To observe the effects of gypenosides （GPS） and ginkgo biloba extract （GBE） mixture on plasma leptin （LP）, adiponectin （APN）, hepatic tissue malondialdehyde （MDA） and superoxide dismutase （SOD） in rats with type 2 diabetes mellitus （T2DM） and non-alcoholic fatty liver disease （NAFLD）. Methods Total of 40 SPF male SD rats （body mass 220~250 g） were randomly divided into blank control group （n = 7） and model group with T2DM and NAFLD （n = 33）. After 8 weeks, the NAFLD model rats were prepared. The model group was then divided into three subgroups： the GPS and GBE mixture intervention group （treatrment group, n = 10） were perfused with 0.5 g/（kg.d） GPS and 0.1 g/（kg.d） GBE mixture, the GPS intervention group （control group, n = 10） were perfused with 0.5 g/（kg.d） GPS, and the model group （n = 10） were perfused with the same volume of water. All the groups were given high fat diet and free drinking, the treatment course was 6 weeks and the expert＇mental period was 14 weeks. The levels of LP and APN in plasma and the MDA and SOD in hepatic tissue were detected. Results （1-）The hepatic tissue MDA level in blank group, model group, treatment group and control group were （3.01± 0.10） nmol/ml, （4.78 ± 0.12） nmol/ml, （3.60 ± 0.09） nmoYml and （4.29 ± 0.11） nmol/ml, respectively. The MDA level in treatment group was lower than that of the control group, with a significant difference （t = 15.35, P = 0.000）. The hepatic tissue SOD level in the blank group, model group, treatment group and control group were （268.4 ± 15.2） U/mt, （140.6 ± 16.8） U/ml, （224.8±15.4） U/ml, and （180.6 ± 16.0） U/ml, respectively. The SOD level in the treatment group was lower than that of the control group, with a significant difference （t = 6.29, P = 0.000）. The plasma APN level in the blank group, model group, treatment group and control group were （7.98 ± 0.96） ng/ml, （4.01 ± 0.88） ng/ml, （5.92 ± 0.12）

关 键 词：绞股蓝皂苷 银杏叶提取物 2型糖尿病 脂肪肝 非酒精性 瘦素 脂联素 丙二醛 超氧化物歧化酶 

分 类 号：R285.5[医药卫生—中药学]