清热解毒化浊片对脂肪性肝病大鼠脂肪因子ADP及炎性因子TNF-α、IL-10的影响  

Effects of Qingre Jiedu Huazhuo tablets on ADP,TNF-α and IL-10 in rat model of fatty liver disease

作　　者：毛娅男[1] 赵国荣[1] 陈研焰 戴玉微 崔玉晖 

机构地区：[1]湖南中医药大学中医学院,湖南长沙410208

出　　处：《中国肝脏病杂志（电子版）》2016年第1期67-71,共5页Chinese Journal of Liver Diseases：Electronic Version

基　　金：湖南省教育厅重点项目(12A101)

摘　　要：目的研究清热解毒化浊片对脂肪性肝病大鼠脂肪因子ADP及炎性因予TNF-α、IL-10的影响。方法采用高脂饮食、酒精灌胃建立脂肪性肝病大鼠模型。造模成功后,随机分为清热解毒化浊片组、非诺贝特组、模型组和正常组4组,每组10只,清热解毒化浊片组按0.081 g/ml灌胃,非诺贝特组按0.083 g/ml灌胃,模型组及正常组予等体积蒸馏水灌胃,每组动物灌胃量均为1 ml/(100 g·d)。经治疗30天后观察肝组织病理学改变,采用全自动生化仪检测血清甘油三酯(TG)、总胆固醇(TC)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)活性,ELISA法检测血清脂联素(ADP)、白细胞介素10(IL-10)、肿瘤坏死因子α(TNF-α)的表达,免疫组织化学二步法测定IL-10、TNF-α在肝组织中的表达。结果光学显微镜下观察,清热解毒化浊片组大鼠肝组织脂肪沉积、炎性浸润情况明显改善。与正常组比较,模型组血清TG、TCHO、ALT、AST、TNF-α显著升高(P值分别为0.000、0.006、0.005、0.005、0.000),血清ADP显著降低(P=0.005),肝组织TNF-α显著升高(P=0.000)。与模型组比较,清热解毒化浊片组血清TG、TCHO、ALT、AST、TNF-α均显著降低(P均为0.000),血清ADP、IL-10显著升高(P=0.02、0.000),肝组织TNF-α显著降低(P=0.000),肝组织IL-10显著升高(P=0.000)。结论清热解毒化浊片可调节肝细胞脂质代谢,减少炎性损伤,其作用机制可能是通过促进脂肪因子ADP的释放,减少脂质堆积,促进抑炎因子IL-10的表达,降低促炎因子TNF-α的释放,从而达到降脂抗炎,保肝护肝的目的。Objective To investigate the effects of Qingre Jiedu Huazhuo tablets on ADP, TNF-α and IL-10 in rat model of fatty liver disease. Methods The rat liver inflammation model was established by feeding high sugar, fat and liquor. The rats were randomly divided into normal group, model group, Fenofibrate group and Qingre Jiedu Huazhuo group, 10 rats in each group. Qingre Jiedu Huazhuo tablets （0.081 g/ml） and Fenofibrate （0.083 g/ml） were used to treat liver inflammatory model rat for 30 days [1 ml/（100g·d）], respectively. The content of blood serum alanine transaminase （ALT）, aspartate transaminase （AST）, triglyceride （TG） and total cholesterin （TC） were measured by colorimetry; tumor necrosis factor-or （TNF〈t）, interleukin-10 （IL-10） and adiponectin （ADP） were measured by enzyme-linked immumsorbent assay （ELISA）. Pathologic changes of liver were measured by a microscope, and the two-step method of immunohistochemistry （IH） was adopted to test the expression of TNF-α and IL-10 in live tissues. Results Light microscopic inspection： Qingre Jiedu Huazhuo tablets can suppress liver inflammation and lipid deposition. The expression of serum TG, TCHO, ALT, AST and TNF〈t in model group were significantly higher than those in normal control group （P = 0.000, 0.006, 0.005, 0.005, 0.000, respectively）. The expression of ADP in model group was significantly lower than that in normal group （P = 0.005）. Compared with the model group, the expression of TG, TCHO, ALT,AST and TNF-u were significantly lower in Qingre Jiedu Huazhuo tablets group （P = 0.000）. The expression of ADP and IL-10 were significantly higher in Qingre Jiedu Huazhuo tablets group （P = 0.02, 0.000）. Qingre Jiedu Huazhuo tablets can significantly reduce the level of TNF-α （P = 0.000） and increase the level of IL-10 （P = 0.000）. Conclusions Qingre Jiedu Huazhuo tablets can regulate lipid metabolism and resist inflammatory injury, which may be associated with the reduced release of pro

关 键 词：清热解毒化浊片 脂肪性肝病 ADP TNF-Α IL-10 

分 类 号：R285.5[医药卫生—中药学]