漆酶的形态特性表征及固定化研究  

Study on morphological characterization and immobilization of laccase

作　　者：张安龙[1] 郗文君 杜飞[1] 周丹妮[1] 杨楠[1] 

机构地区：[1]陕西科技大学陕西省造纸技术及特种纸品开发重点实验室,陕西西安710021

出　　处：《陕西科技大学学报（自然科学版）》2016年第2期6-11,共6页Journal of Shaanxi University of Science & Technology

基　　金：国家十二五科技支撑计划项目(2011BAC11B04);陕西科技大学博士科研启动基金项目(BJ14-09)

摘　　要：使用固定化漆酶进行造纸废水深度处理可显著减少水中残留木素含量,运用SEM、XRD、激光粒度、能谱等分析手段,可提供一种关于漆酶固定化效果的有效评价方法.漆酶分子粒径主要集中在11.482～19.953μm,海藻酸钠法及环氧化PVA法对漆酶的固定方式分别为内部包埋和表面吸附.在海藻酸钠包埋法中,固定的酶分子粒径大、数量多、固定牢固而不易脱落;环氧化PVA趋向于吸附小粒径的漆酶分子、易受环境影响,固定效果差.在相同活力下,海藻酸钠固定化漆酶结晶度高于PVA吸附法.硫、钠元素的定性,证实了漆酶的固定化效果.The use of immobilized laccase in papermaking wastewater treatment can significantly reduce the residual lignin content in water.The use of SEM,XRD,laser particle size,energy spectrum analysis can provide an effective evaluation method for laccase immobilization.The molecular size of the laccase was mainly concentrated in the 11.482～19.953μm.The principle for immobilization of laccase by sodium alginate and PVA were internal embedding and surface adsorption respectively.The particle size and the number of the enzyme molecules in the sodium alginate embedding method were large and fixed firmly and not easy to fall off;Epoxidized PVA immobilized laccase tended to absorb small particles and vulnerable to environmental impact,so the fixed affect was poor.Under the same activity,the crystallinity of the immobilized laccase by sodium alginate was higher than that of the PVA adsorption method.The qualitative analysis of the sulfur and sodium elements confirmed the effect of laccase immobilization.

关 键 词：木质素 固定化漆酶 SEM XRD 形态分析 

分 类 号：X793[环境科学与工程—环境工程]