稀土元素对他克莫司生物合成和相关基因表达的影响  

Effect of Rare Earth Elements on the Biosynthesis of Tacrolimus and Related Gene Expression

作　　者：孙燕[1] 叶蕊芳[1] 任雪岩 

机构地区：[1]华东理工大学生物工程学院,上海200237

出　　处：《中国医药工业杂志》2016年第4期402-406,共5页Chinese Journal of Pharmaceuticals

摘　　要：他克莫司(1)作为一种广泛使用的免疫抑制剂,是由筑波链霉菌(Streptomyces tsukubaensis)发酵产生的。本试验通过在1生物合成过程中添加稀土元素,考察氯化镧、氯化铕和氯化铯对筑波链霉菌No.9993生物合成1的影响,并从添加氯化镧后其相关基因转录水平表达量的角度进行分析。结果显示,在发酵96 h添加10.0 mg/L氯化镧,1产量较对照提高37.5%,1生物合成过程中的结构基因fkb P的表达量显著增加,fkb O表达量也略有增加。提示氯化镧对1生物合成相关基因的转录有正向调控作用。Tacrolimus （1）, a widely used immunosuppressant, is produced by a fermentative strain Streptomyces tsukubaensis. Three rare earth elements, lanthanum （LaCl3）, scandium （EuC13） and europium （CsCI）, were added during the biosynthetic process to investigate their effects on the production of 1 by S. tsukubaensis No.9993, and the expression of the related genes after adding LaCl3 was analyzed. The results showed that the productivity of 1 increased 37.5 % compared with the control group when 10.0 mg/L LaCl3 were added at 96 h. The expression of structural genefkbP was remarkablely increased, while the expression of fkbO enhanced slightly. It suggested that lanthanum might play a positive role on the transcription of 1 structural gene.

关 键 词：他克莫司 稀土元素 RT-PCR 基因转录 

分 类 号：Q939.9[生物学—微生物学]