针刺对卒中后抑郁大鼠空间学习记忆功能及海马CA3区脑源性神经营养因子的影响  

Effect of Acupuncture on Spatial Learning and Memory Abilities and Hippocampal CA3 Regional BDNF in Rats with Post-stroke Depression

作　　者：蔡丽[1] 刘毅[1] 陆小青[1] 

机构地区：[1]上海中医药大学附属市中医医院,上海200071

出　　处：《上海针灸杂志》2016年第4期462-465,共4页Shanghai Journal of Acupuncture and Moxibustion

基　　金：上海市教委预算内项目(2012JW60);上海市青年医师培养项目;上海市中医医院优青项目

摘　　要：目的观察针刺百会、印堂、四神聪穴对卒中后抑郁(PSD)模型大鼠空间学习记忆功能及海马区脑源性神经营养因子(BDNF)表达水平的影响。方法将30只成年雄性SD大鼠,按随机数字表随机分为对照组、针刺组及假手术组,每组10只。采用经典的线栓大脑中动脉阻塞(MCAO)法加用慢性不可预见温和应激(CUMS)结合孤养建立大鼠PSD模型。于应激开始第14天开始,针刺组予以针刺干预,连续干预14 d。应激结束后1星期进行Morris水迷宫试验,评价大鼠空间学习与记忆功能。水迷宫测试结束后用免疫组化法测定海马CA3区BDNF的表达。结果与假手术组比较,对照组大鼠学习能力显著下降(P<0.05),反映记忆功能的空间探索时间和跨平台次数均显著降低,分别是(22.36±6.20)s与(48.73±5.40)s、(3.72±0.90)次与(8.60±1.10)次,差异具有统计学意义(P<0.01)。PSD大鼠经针刺治疗后,与对照组比较,学习能力及空间探索时间和跨平台次数、BDNF表达均显著提高,差异具有统计学意义(P<0.01)。结论针刺治疗可改善卒中后抑郁大鼠的空间学习记忆功能,提高卒中后抑郁大鼠模型海马区BDNF水平。Objective To investigate the effects of acupuncture at points Baihui（GV20）,Yintang（Ex-HN3）and Sishencong（Ex-HN1）on spatial learning and memory abilities and hippocampal expression of brain-derived neurotrophic factor（BDNF）in a rat model of post-stroke depression（PSD）.Method Thirty male adult SD rats were allocated,using a random number table,tocontrol,acupuncture and sham operation groups,10 rats each.A rat model of PSD was made by classical middle cerebral artery occlusion（MCAO）using an intraluminal thread technique,chronic unpredictable mild stress（CUMS）and solitary feeding.At the fourteenth day after the beginning of stress,the acupuncture group received acupuncture intervention for 14 consecutive days.At one week after the end of stress,the Morris water maze test was conducted to assess rat spatial learning and memory abilities.After the completion of the water maze test,hippocampal CA3 regional BDNF expression was determined by immunohistochemical method.Result Rat learning and memory abilities decreased significantly in the control group compared with the sham operation group（P〈0.05）.Space exploration time and the number of crossing platform reflecting memory abilities decreased significantly in the control group（22.36±6.20 s and 3.72±0.90 times,respectively）compared with the sham operation group（48.73±5.40 s and 8.60±1.10 times,respectively）;there were statistically significant differences（P〈0.01）.Learning abilities,space exploration time,the number of crossing platform and BDNF expression increased significantly in PSD rats treated with acupuncture compared with the control group;there were statistically significant differences（P〈0.01）.Conclusion Acupuncture can improve spatial learning and memory abilities and raise hippocampal regional BDNF levels in rats with post-stroke depression.

关 键 词：针刺 中风并发症 抑郁症 脑源性神经营养因子 记忆障碍 大鼠 

分 类 号：R2-03[医药卫生—中医学]