2009—2012年广东省发热呼吸道综合征病毒性病原谱分析  

Analysis of viral pathogenic spectrum of febrile respiratory syndrome in Guangdong Province in 2009—2012

作　　者：王雅静[1] 武婕[2] 康敏[3] 郑小凌[1] 王冰姝[1] 钟昱文[1] 陈惠珍[1] 沈秀婷[1] 

机构地区：[1]广东省疾病预防控制中心消毒与病媒生物预防控制所,广州511430 [2]广东省疾病预防控制中心病原微生物所,广州511430 [3]广东省疾病预防控制中心传染病预防控制所,广州511430

出　　处：《广东医学》2016年第8期1197-1202,共6页Guangdong Medical Journal

基　　金：广东省科技计划项目(编号:2013B020307006);广州市科技计划项目(编号:2014J4100091)

摘　　要：目的分析广东省发热呼吸道综合征病毒性病原谱,为了解呼吸道病毒在广东省人群中的流行进化规律、提高广东省不明原因肺炎的临床诊疗及疫苗的研发提供重要的实验室依据。方法收集2009—2012年广东省东西南北中5个城市的1 484份住院发热呼吸道标本,应用自行设计的实时荧光定量PCR引物和探针,对流感病毒（Flu）、腺病毒（ADV）、呼吸道合胞病毒（RSV）、偏肺病毒（MPV）、副流感病毒（PIV）、博卡病毒（Bo V）和冠状病毒（Co V）等7种常见呼吸道病毒进行检测。结果 Flu是这4年广东省人群发热呼吸道综合征的主要病毒性病原体,有季节分布规律（2—3月、7—8月和11—12月高发）和年龄分布规律（在〈1岁的婴儿中高发）,但没有明显的性别分布差异。ADV、RSV、PIV、MPV和Bo V这5种病毒的阳性率均没有明显的季节分布规律和性别差异,但有年龄分布特征：ADV在〈1岁的婴儿中高发,RSV、PIV、MPV和Bo V这4种病毒均在1-2岁的幼儿中高发。Co V没有明显的年龄分布特征,但有年份分布特征,阳性率从2009—2012年呈现逐年上升的趋势。这7种病毒均存在交叉感染现象,Bo V的交叉感染率最高,Flu的交叉感染率最低。结论广东省发热呼吸道综合征存在多种病毒性病原体,ADV、RSV、MPV、PIV和Bo V这5种病毒在广东省这4年的进化处于平缓阶段,而Flu和Co V每年均在变化,需加强多种呼吸道病毒的监测。Objective To analyze the viral pathogenic spectrum of febrile respiratory syndrome（ FRS） in Guangdong Province,in order to provide an important experimental basis for understanding the epidemic evolutionay pattern of respiratory viruses in Guangdong population,and to improve the clinical diagnosis and vaccine development for unexplained pneumonia in Guangdong Province. Methods 1 484 specimens from subjects with FRS were collected from 8general hospitals in 5 cities of Guangdong Province from 2009 to 2012. Influenza virus（ Flu）,adenovirus（ ADV）,respiratory syncytical virus（ RSV）,metapneumovirus（ MPV）,parainfluenza virus（ PIV）,Boca virus（ Bo V） and Corona virus（ Co V） were detected using q PCR with self- designed primers and probes. Results The Flu was the main virulent pathogen of FRS in Guangdong Province over these 4 years,and had an obvious seasonal and age distribution： high prevalence from February to March,from July to August,from November to December,and in babies aged less than 1 year,without gender difference. There was age distribution while no seasonal distribution or gender difference among ADV,RSV,PIV,MPV and Bo V： ADV had high prevalence in babies aged less than 1 year. RSV,PIV,MPV and Bo V had high prevalence in infants aged from 1 to 2 years. The positive rate of Co V increased year by year from 2009 to 2012 while it had no age distribution. Coinfection of one or more of the 7 viruses were detected. The coinfection rate of Bo V was the highest while that of Flu was the lowest. Conclusion There are many types of viral pathogens in FRS in Guangdong Province. The evolution of ADV,RSV,PIV,MPV and Bo V in Guangdong Province during the 4 years is in a gradual phase,while Flu and Co V change every year. The monitoring of a variety of respiratory viruses should be strengthened.

关 键 词：呼吸道病毒 发热 H1N1pdm09流感病毒 

分 类 号：R372[医药卫生—病原生物学]