纳米金比色法测定多西环素和土霉素  

Highly sensitive colorimetric detection on doxycycline and oxytetracycline based on gold nanoparticles

作　　者：曲黎[1] 魏燕丽[1] 范淑敏[1] 

机构地区：[1]河南科技学院新科学院,河南新乡453003

出　　处：《化学研究》2016年第2期189-194,共6页Chemical Research

基　　金：国家自然科学基金(51304064)

摘　　要：采用柠檬酸钠还原法合成粒径约13nm的纳米金粒子.采用紫外-可见分光光度计、荧光分光光度计研究了纳米金粒子与多西环素/土霉素分子的相互作用;通过改变缓冲溶液、纳米金粒子用量、反应时间确定了比色法测定的最优反应条件.结果表明:在弱酸溶液中,多西环素/土霉素分子中的氨基官能团(-NH_2)得到电子成为带电基团(-NH_3^+)并通过静电引力与纳米金粒子结合,使得纳米金粒子发生聚集,导致纳米金吸收光谱发生红移和展宽,颜色由酒红色变成蓝色;在盐酸-柠檬酸钠的缓冲溶液中加入2mL纳米金,反应时间为10min的条件下测得多西环素和土霉素的线性范围分别为0.06~0.66mg·L^(-1)和0.59~8.85 mg·L^(-1),检出限(3σ)分别为0.008 6、0.083 8mg·L^(-1).该方法前处理简单、灵敏、可靠,有望应用于食品分析和临床分析等领域.The well-dispersed gold nanoparticles（GNPs）were synthesized through sodium citrate reduction method.UV-Vis spectrometer and fluorescence spectrophotometer were used to investigate the mutual effect between GNPs and doxycycline/oxytetracycline.The experimental parameters were optimized with regard to pH,incubation time and concentration of the GNPs.The amino group,which is positively charged in weakly acidic medium（namely,-NH3-＋）,integrates with the negative charge on the surface of gold nanoparticles to form a bigger binding product through electrostatic binding,resulting the aggregation of GNPs with a red-to-purpleto-blue color change.The absorption band of GNPs had a red shift and broadened.Under optimal experimental conditions,the linear ranges of the colorimetric sensor were 0.06-0.66mg·L^-1 and 0.59-8.85 mg·L^-1 with the corresponding limit of detection（3σ）of 0.008 6and0.083 8mg·L^-1 for doxycycline and oxytetracycline respectively.This method is promising for foods and clinical analysis owing to to its simple,reliable,convenient and low-cost.

关 键 词：纳米金粒子 比色法 多西环素 土霉素 

分 类 号：O652.7[理学—分析化学]