美国黑桦、欧洲桤木和川桤木对重金属Cu胁迫的响应研究  

Response of Betula nigra, Alnus glutinosa and Alnus crmastogyme to Heavy Metal Cu Stress

作　　者：张志超[1,2] 饶龙兵[2] 段红平[1] 

机构地区：[1]云南农业大学资源与环境学院,昆明650201 [2]中国林业科学研究院亚热带林业研究所,浙江富阳311400

出　　处：《中国农学通报》2016年第10期13-23,共11页Chinese Agricultural Science Bulletin

基　　金："十二五"国家科技支撑项目桤木育种专题"抗逆生态树种桤木新品种选育"(2012BAD01B0604);中央级公益性科研院所基本科研业务费专项基金"不同倍性桤木遗传变异研究"(RISF2013010)

摘　　要：为了研究美国黑桦、欧洲桤木和川桤木3种植株对Cu胁迫的响应以及3个树种间的耐Cu性差异。通过盆栽试验的方法研究不同浓度Cu处理下的3种植株的生长、抗氧化酶系统的变化以及Cu积累特征。研究结果表明:(1)高浓度Cu(≥1 mmol/L)处理显著降低3种植株的株高、地茎生长量以及生物量,抑制植株的生长;(2)随Cu胁迫时间的延长,幼苗叶片SOD、POD、CAT活性均呈先升高后下降的趋势,欧洲桤木中3种酶活性更强,且酶活性持续时间最长;(3)川桤木和欧洲桤木根、茎、叶Cu积累量均随处理浓度的升高而增加,美国黑桦根、茎Cu积累量随处理浓度升高而增加,而叶片Cu积累量随处理浓度升高而减少。高浓度Cu处理对3种植株的生长都有显著的抑制作用,3种植株的都表现出对Cu离子的根部富集特征。欧洲桤木整体耐Cu能力最强,美国黑桦在Cu浓度较低时有较强的耐Cu能力,川桤木对Cu胁迫相对比较敏感。In order to study the response of Betula nigra, Alnus glutinosa and Alnus crmastogy me to Cu stress and the difference of Cu tolerance among the three species, plant growth, changes of antioxidant system and Cuaccumulation characteristics under different Cu concentrations of these three species were studied by themethod of pot experiment. The results showed that（1） high Cu concentration（≥1 mmol/L） significantly reducedplant height, stem increment and biomass, which inhibited plant growth;（2） with the increase of Cu stress time,the activities of SOD, POD and CAT increased and then decreased, these three enzymatic activities in Alnus glutinosa were the strongest and the longest;（3） Cu accumulation in Alnus glutinosa and Alnus crmastogyme root, stem and leaf increased with the increase of Cu concentration, Cu accumulation in Betula nigra root andstem increased but Cu accumulation in leaf decreased with the increase of Cu concentration. In summary, plantgrowth of the three species was inhibited under high Cu concentration, they all showed Cu2 ＋enrichmentcharacteristics in root. The Cu tolerance of Alnus glutinosa was the strongest, Betula nigra had strong Cu resistance when the concentration of Cu was low, Alnus crmastogy me was relatively sensitive to Cu stress.

关 键 词：美国黑桦 桤木 CU胁迫 

分 类 号：S792.14[农业科学—林木遗传育种]