辐射诱发兰花叶艺突变体的叶片叶肉细胞超微结构观察  

Ultrastructure of Mesophyll Cell in Cymbidium with Verge Line Pattern Lives Induced Mutation by Radiation

作　　者：苏畅[1] 李枝林[1] 李夏媛 王玉英[1] 

机构地区：[1]云南农业大学园林园艺学院花卉研究所,云南昆明650201

出　　处：《热带农业科学》2016年第3期24-27,共4页Chinese Journal of Tropical Agriculture

基　　金：国家自然科学基金项目(No.30160074);云南省自然科学基金重点项目(No.2002C0003P);云南省重点新产品开发项目(No.2012BB008);云南农业大学园林园艺学院院级基金(No.2014001);云南省昆明市科学技术局重点项目(No.2015-1-N-00984)

摘　　要：以未经辐射的全绿植株(TRIR-1)和经辐射诱发叶艺兰植株(TRIR-2)的叶肉细胞为试材,对其全绿植株叶片的正常绿色区、辐射诱变植株叶片的绿色区(TRIR-2′)和白色区(TRIR-2″)叶片叶肉细胞进行细胞超微结构观察。结果表明:(1)TRIR-1叶肉细胞中叶绿体数量最多,TRIR-2′次之,TRIR-2″中无成熟完整的叶绿体;(2)TRIR-1叶肉细胞中的线粒体呈椭圆颗粒状,TRIR-2′中的线粒体外膜破裂、线粒体溶解、嗜锇颗粒数量最多,TRIR-2″中有少量外膜完整的线粒体;(3)TRIR-2″部分叶肉细胞中无细胞器,只有少量细胞质。说明经辐射诱变叶艺兰与未经辐射植株叶肉细胞结构存在差异。The test materials of this study are mesophyll cells which take from cymbidium without induced by irradiation（TRIR-1） and new mutants induced by irradiation（TRIR-2）, including normal green area from TRIR-1 and both green area（TRIR-2′） and the white area（TRIR-2″） from TRIR-2.Ultrastructure of mesophyll cells were observed by Transmission Electron Microscope（TEM）. The results showed that：（1） In the mesophyll cells, the quantity of chloroplasts in TRIR-1 was the most, the quantities of chloroplasts in TRIR-2′ and TRIR-2″ were less. There are not even a little bit mature and complete chloroplast in the mesophyll cells of TRIR-2″;（2） The mitochondria in cell of TRIR-1 showed ellipsoid,mitochondrial membrane ruptured and mitochondria dissolved in TRIR-2′ the content of osmophilic gramules is the most in the cell of TRIR-2′, a small amount mitochondria with intact membrane appeared in TRIR-2″;（3） There is nothing but a few cytoplasm in some cell of TRIR-2″. This shows that some difference between the cymbidium without induced by irradiation and new mutants induced by irradiation in cell ultrastructure.The result can provide evidence for variety breeding of Cymbidium with verge line pattern lives.

关 键 词：黄色素花虎头兰 黄蝉兰 叶肉细胞 超微结构 叶绿体 线粒体 辐射诱变 

分 类 号：S682.31[农业科学—观赏园艺]