两株H1亚型禽流感病毒的全基因组序列测定及其对小鼠的感染性研究  

Evolution and infectivity in mice of two H1 subtype avian influenza viruses

作　　者：彭志[1,2] 崔鹏飞[2] 葛叶[2] 关立峥[2] 肖丽[2] 余兴龙[1] 邓国华[2] 陈化兰[2] 

机构地区：[1]湖南农业大学,湖南长沙410128 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/农业部动物流感重点实验室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2016年第4期271-274,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家科技攻关项目(KJYJ-2013-01-01)

摘　　要：为了解H1亚型禽流感病毒(AIV)的生物学特性,本研究对2015年从我国南方活禽市场分离到的两株鸭源H1亚型AIV A/duck/Hu N/S10153/2015(H1N1)(简称Hu N/153/15)和A/duck/JX/S11233/2015(H1N3)(简称JX/1233/15)进行全基因组序列的测定及进化分析,并对其进行BALB/c小鼠的感染性试验。氨基酸序列分析显示:两株病毒HA裂解位点均只含有一个碱性氨基酸,具有低致病性AIV(LPAIV)的分子特征,其中Hu N/153/15的内部基因片段来源复杂,呈现明显的遗传多样性。小鼠感染性试验结果显示,Hu N/153/15能够在小鼠的肺脏和鼻甲中有效复制,而JX/1233/15在小鼠的各个脏器中均未检测到病毒的复制,表明其暂时还不具备感染哺乳动物的能力。本研究结果为H1亚型AIV的持续性监测和相关生物学特性的研究奠定基础。To investigate the biological characteristics of H1 subtype avian influenza viruses（AIVs） in China, we sequenced the whole genome and evaluated the infectivity in BALB/c mice of two H1 subtype AIVs A/duck/Hu N/S10153/2015（H1N1）（Hu N/153/15） and A/duck/JX/S11233/2015（H1N3）（JX/1233/15） which were isolated from live-poultry markets in 2015. Sequence analysis showed that the two isolates only have one basic amino acids at HA cleavage site, which was a characteristic of low pathogenesis avian influenza virus（LPAIV）, and the internal genes of Hu N/153/15 showed distinct diversity. The infectious experiments in BALB/c showed that Hu N/153/15 was able to replicate efficiently in nasal turbinate and lung of mice, but JX/1233/15 were not detected in any organs of mice, indicating the isolate had no ability to infect mammal.

关 键 词：禽流感病毒 H1N1 H1N3 进化分析 感染性 

分 类 号：S852.65[农业科学—基础兽医学]