IGF-1基因多态性与猪生长性状的相关性分析  

Association between the IGF-1 Gene Polymorphism and Growth Traits in Pigs

作　　者：许金根[1,2] 陶立[2] 王立克[1] 王重龙[2] 刘林清[2] 李庆岗[2] 

机构地区：[1]安徽科技学院动物科学学院,安徽凤阳233100 [2]安徽省农业科学院畜牧兽医研究所,安徽合肥230031

出　　处：《安徽科技学院学报》2016年第1期7-11,共5页Journal of Anhui Science and Technology University

基　　金：安徽省教育厅自然科学研究重点项目(KJ2016A180);安徽省农业科学院杰出青年创新基金项目(14B0404);安徽省自然科学基金青年项目(1608085QC72)

摘　　要：目的:研究淮猪新品系与杜洛克猪杂交的生产性能,并分析IGF-1基因对猪生长性状的遗传效应。方法:通过ACEMA-64自动饲喂系统测定杜洛克猪、淮猪新品系、杜洛克猪×淮猪新品系的生长性状。另外,用PCR-RFLP方法对测定猪的基因多态性进行检测。结果:杜洛克猪、淮猪新品系×杜洛克猪的平均日增重和达100kg校正日龄都显著优于淮猪新品系(P<0.05);淮猪新品系×杜洛克猪的饲料转化率显著低于淮猪新品系(P<0.05),但也显著高于杜洛克猪(P<0.05)。IGF-1基因PCR产物经Hha I酶切,杜洛克猪仅检测到BB型;淮猪新品系检测到AB和BB型;杜洛克猪×淮猪新品系检测到AA、AB和BB型。关联分析表明,三种基因型个体的平均日增重、饲料转化率和达100kg校正日龄存在差别,但是差异都不显著(P>0.05)。其中,淮猪新品系BB型个体的生长性状有优于AB型的趋势,杜洛克猪×淮猪新品系BB型个体有优于AB和AA型的趋势。结论:淮猪新品系与杜洛克猪杂交效果较好,但IGF基因作为改善猪生长性状的分子标记仍需进一步的验证。Objective： To explore the production performance of Huai pig hybrided with Duroc pigs,and to analyze the genetic effect of the IGF- 1gene on growth traits in pigs. Method： Growth traits of Duroc pigs,Huai pig and Duroc pigs × Huai pig were measured by the automatic feeding system of ACEMA64. In addition,the IGF- 1 gene polymorphism was detected by polymerase chain reaction- restriction fragment length polymorphism（ PCR- RFLP） method in these tested pigs. Results： The average daily gain and corrected day at 100 kg of Duroc pigs and Duroc pigs × Huai pig were significantly better than Duroc pigs × Huai pig（ P〈0. 05）; the feed conversion rate of Duroc pigs × Huai pig was significantly lower than Huai pig（ P〈0. 05）,but was significantly higher than Duroc pigs（ P〈0. 05）. PCR products of the IGF- 1 gene were digested with the Hha I enzyme,and only BB genotype was detected in Duroc pigs; AB and BB genotypes were detected in Huai pig; AA,AB and BB genotypes were detected in Duroc pigs × Huai pig. Association analysis showed that the differences were existedin the average daily gain,feed conversion rate,and corrected day at 100 kg among three genotype individuals,but the differences were not significant（ P〈0. 05）. Among them,the growth traits of individuals with the BB genotype had a better trend than that of AB genotype in Huai pig new line,and individuals with the BB genotype had a better trend than that of AB and AA genotypes in Duroc pigs × Huai pig new line. Conclusion： Huai pig new line and Duroc pigs have a good hybrid effect,but the IGF- 1 gene is taken as a molecular marker for improving the growth traits of pigs still needs further verification.

关 键 词：猪 IGF-1 基因多态性 生长性状 

分 类 号：S813.3[农业科学—畜牧学]