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机构地区:[1]新疆大学离子束生物工程中心,新疆乌鲁木齐830046
出 处:《北方园艺》2016年第8期130-132,共3页Northern Horticulture
基 金:新疆维吾尔自治区青年科技创新人才培养工程资助项目(2013721007)
摘 要:以阿魏菇多糖高产菌PFPH-2为试验菌株,采用营养因子正交优化及发酵过程动态培养方法,研究营养因子对PFPH-2菌丝体多糖产量的影响以及动态发酵过程PFPH-2菌丝体生物量与发酵液pH值的相关性。结果表明:正交优化组合对PFPH-2菌丝体胞内多糖积累有协同效应,在最佳组合的发酵体系中PFPH-2菌丝体胞内多糖产量达到(903.73±1.23)mg/L的最高水平;发酵体系中PFPH-2菌丝体生物量与发酵液pH值有一定相关性,以发酵液pH值降到低点且趋于稳定的时间终止发酵其菌丝体生物量最大。Taking Pleurotus polysaccharide high-yield strain PFPH-2as experimental strain,the influence of nutrition factor on mycelial polysaccharide yield of PFPH-2as well as the relevance of mycelium biomass of PFPH-2with the pH value of fermented broth were studied through the method of nutrition factor orthogonal optimization and dynamic fermentation.The results showed that the orthogonal combination indicated a synergistic effect on the accumulation of mycelium polysaccharides of PFPH-2.In the optimal combination fermentation medium,the yield of mycelium polysaccharides of PFPH-2increased to(903.73±1.23)mg/L by the end of fermentation.Mycelium biomass had a certain correlation with the pH of fermentation broth in fermentation system of PFPH-2.The mycelium biomass achieved the maximum amount when the pH values of fermentation broth down to low and tend to stabilization.
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