半滑舌鳎生长相关的微卫星标记及优势基因型  

Analysis of Significant Correlation Microsatellite Marker and Preferred Genotypes with Growth Traits in Half-smooth Tongue Sole(Cynoglossus semilaevis)

作　　者：鲁翠云[1] 李超[1] 郑先虎[1] 曹顶臣[1] 程磊[1] 崔恒全 毛成全 孙效文[1] 

机构地区：[1]中国水产科学研究院黑龙江水产研究所淡水鱼类育种国家地方联合工程实验室,黑龙江哈尔滨150070 [2]海阳市黄海水产有限公司,山东海阳265122

出　　处：《水产学杂志》2016年第2期1-8,共8页Chinese Journal of Fisheries

基　　金：山东泰山学者蓝色产业领军人才团队支撑计划项目

摘　　要：本研究利用SPSS 19.0的GLM模型分析28个微卫星标记与半滑舌鳎Cynoglossus semilaevis生长性状的相关性,结果获得14个与半滑舌鳎体质量(BWe)、体长(BL)或体宽(BWi)显著相关的微卫星标记。与雄性个体生长性状显著相关的7个标记中,cse56与体质量、体长和体宽3个性状均显著相关(P<0.05);csou24、hncyse68、hncyse147和hncyse148与体质量和体宽显著相关(P<0.05)。与雌性个体生长性状显著相关的10个标记中,cse151和csou56与3个性状均显著相关(P<0.05);csou33、csou39和hncyse108等5个标记与其中2个性状显著相关(P<0.05)。csou24、hncyse147和hncyse148在雄、雌群体中均表现出与生长性状的相关性。本研究共筛选出17个对雄性个体生长具有显著正面影响的优势基因型,29个雌性个体的优势基因型。In this study, the correlation was analyzed between 28 microsatellite markers and growth traits in half-smooth tongue sole（ Cynoglossus semilaevis） using the GLM procedure of SPSS 19.0. As a result, a total of fourteen markers had a significant correlation between the genotype and body weight（ BWe）, body length（ BL）, and/or body width（ BWi）. In the seven significant markers in male,one marker（ cse56） were significantly associated with BWe, BL, and BWi（ P〈0.05）; four markers（ csou24, hncyse68, hncyse147, and hncyse148） were significantly associated with BWe and BWi（ P〈0.05）. In female, there were ten markers that had a significant impact on growth traits, in which, cse151 and csou56 had a significant impact on BWe, BL, and BWi; five markers including cse120, csou33,csou39, hncyse108, and hncyse147 showed a significant impact on the two traits. It was worth mentioning that three markers including csou24, hncyse147 and hncyse148 were shared in male and female associated with growth traits. In addition, seventeen preferred genotypes were detected using Duncan multiple comparisons in male and twenty-nine preferred genotypes in female. The findings will be useful for a marker-associated selection breeding program in half-smooth tongue sole.

关 键 词：半滑舌鳎 微卫星标记 优势基因型 

分 类 号：S917[农业科学—水产科学]