酸性条件对旋毛虫肌幼虫TsGAD mRNA表达水平的影响  

Effect of different acid environment on expression of TsGAD mRNA in Trichinella spiralis muscle larvae

作　　者：王泽[1] 胡静[1] 许静秀 李晓云[1] 刘畅[1] 赵祥[1] 王琦[1,2] 宋铭忻[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030 [2]鹤岗出入境检验检疫局,黑龙江鹤岗154100

出　　处：《中国兽医科学》2016年第4期467-472,共6页Chinese Veterinary Science

基　　金：家畜疫病病原生物学国家重点实验室开放基金课题(SKLVEB2015KFKT010);国家自然科学基金项目(31172312)

摘　　要：为探讨旋毛虫肌幼虫谷氨酸脱羧酶(TsGAD)在酸性条件下的抗酸作用,应用实时荧光定量PCR技术对不同酸性条件下培养的TsGAD mRNA的表达水平进行检测。结果显示,随着pH值的降低,TsGAD mRNA表达量逐渐升高,在pH 2.5时TsGAD mRNA表达量显著高于pH 4.0、pH 6.6和pH 9.0(P<0.05)。结果表明,强酸环境能显著诱导TsGAD mRNA的表达,旋毛虫肌幼虫具有谷氨酸依赖型抗酸机制,这为进一步研究旋毛虫的抗酸机理提供了基础数据。To explore the role of Trichinella spiralis muscle larvae glutamate decarboxylase（TsGAD）in T.spiralis muscle larvae acid resistance mechanism,we detected the expression level of TsGAD mRNA in different acid cultivation environment by real-time PCR. As the results showed,with the decrease of the pH value,the expression level of TsGAD mRNA increased gradually. The expression level of TsGAD mRNA in pH 2.5 cultivation environment was statistically higher than that in pH 4.0,pH 6.6 or pH 9.0（P〈0.05）. This study confirmed the exists of a glutamate decarboxylase mediated acid resistance mechanism in T.spiralis muscle larvae and acid cultivation environment could facilitate the expression level of TsGAD mRNA,which laid the foundation for further studies on the acid resistance mechanism in the parasite.

关 键 词：旋毛虫 肌幼虫 谷氨酸脱羧酶(GAD) pH 实时荧光定量PCR 

分 类 号：S852.731[农业科学—基础兽医学]