色氨酸在自制人血清白蛋白手性柱上的拆分及手性识别机理研究  

Enantioseparation of Tryptophan on Human Serum Albumin-immobilized Chiral Stationary Phase and Study on the Mechanism of Chiral Recognition

作　　者：林艳萍[1] 亢珊珊[1] 姚碧霞[1] 翁文[1] 

机构地区：[1]闽南师范大学化学与环境学院,福建漳州363000

出　　处：《福建分析测试》2016年第2期7-10,共4页Fujian Analysis & Testing

基　　金：福建省自然科学基金(2012J06005);福建省质量技术监督局项目(fjqi2013109)资助

摘　　要：人血清白蛋白与三氯聚氰活化的氨丙基硅胶反应,制得人血清白蛋白键合手性固定相。反相模式下,色氨酸在该手性固定相上获得理想的拆分,分离因子可达3.51,分离度达5.49。探讨了流动相p H值、有机修饰剂、柱温等对手性拆分的影响。通过前沿分析法对色谱保留机理进行了探讨。Human serum albumin（HSA）based chiral stationary phase（CSP）was conveniently prepared by HSA and 2,4,6-trichloro-1,3,5-triazine activated aminopropyl silica gel. Tryptophan was enantioseparated successfully on the as-prepared CSP under the reverse-phase mode. The separation factor and the resolution reached 3.51 and 5.49,respectively.The effects of p H value of mobile phase,type and content of organic modifier,and column temperature on the enantioseparation of tryptophan were investigated. The mechanism of chiral recognition was studied further with frontal analysis method.

关 键 词：手性固定相 手性拆分 人血清白蛋白 色氨酸 前沿分析法 

分 类 号：O657.7[理学—分析化学]