冬虫夏草化学成分分析  

Analysis of chemical compounds in Chinese cordyceps

作　　者：钱正明 李文庆 孙敏甜 刘杏忠[2] 李二伟[2] 李文佳 

机构地区：[1]广东东阳光药业有限公司国家中医药管理局重点研究室,广东东莞523850 [2]中国科学院微生物研究所真菌学国家重点实验室,北京100101 [3]宜昌山城水都冬虫夏草有限公司,湖北宜昌443000

出　　处：《菌物学报》2016年第4期476-490,共15页Mycosystema

基　　金：国家中医药管理局中医药科学技术研究专项;中药饮片质量保障体系研究(一)(201507002);东莞市第二批引进创新科研团队(201460720200028)~~

摘　　要：本研究建立了冬虫夏草中7类主要化学成分的分析方法,包括采用高效液相色谱法分析冬虫夏草中的多糖、糖醇、氨基酸和核苷,采用气相色谱法分析冬虫夏草中的甾醇、脂肪酸和挥发性成分,所建立的分析方法均成功运用于冬虫夏草样本分析。从冬虫夏草样本中鉴定了61个小分子化合物(2个糖醇、16个氨基酸、9个核苷、7个甾醇、18个脂肪酸、9个挥发性成分);在对其多糖分析中发现其分子量主要分布于3.3–1 300k Da范围内,由3个主要色谱峰组成。同时,还比较分析了培植冬虫夏草和野生冬虫夏草的化学成分,结果显示两者小分子化合物种类一致,多糖分子量分布一致。The chemical compounds in Chinese cordyceps including polysaccharides, sugar alcohols, amino acids and nucleosides were analyzed by HPLC, and sterols, fatty acids and volatile compounds by GC. A total of 61 small molecule compounds including 2 sugar alcohols, 16 amino acids, 9 nucleosides, 7 sterols, 18 fatty acids and 9 volatile chemicals were identified. Three main polysaccharide peaks were detected with the molecule weight between 3.3KDa and 1 300 KDa. The comparison of wild Chinese cordyceps strains and cultivated ones showed that all kinds of small molecule compounds and the pattern of the polysaccharides were similar, indicating that there is no significant difference between the natural strains and cultivated ones.

关 键 词：化学成分 野生冬虫夏草 培植冬虫夏草 高效液相色谱 气相色谱 

分 类 号：S567.35[农业科学—中草药栽培] R284.1[农业科学—作物学]