溶磷红豆草根瘤菌的分离和筛选  

Isolation and Screening of Phosphate Solubilizing Rhizobia of Sainfoin

作　　者：李剑峰[1,2] 张淑卿[1,2] 师尚礼[2] 杜建雄[3] 李丽霞[1] 

机构地区：[1]贵州师范学院喀斯特生境土壤与环境生物修复研究所,贵州贵阳550018 [2]甘肃农业大学草业生态系统教育部重点实验室,甘肃兰州730070 [3]贵州财经大学,贵州贵阳550025

出　　处：《草地学报》2016年第1期218-221,共4页Acta Agrestia Sinica

基　　金：国家自然科学基金项目(31300389;41561072);贵州省科技基金项目(黔科合J字[2014]2137);贵州省科技基金项目(黔科合J字[2014]2136)资助

摘　　要：为获得溶磷红豆草（Onobrychis viciaefolia）根瘤菌（Rhizobium sp.）,本研究以Winogradsky无氮培养基和PKO溶磷培养基从红豆草根瘤中分离菌株。按解磷-固氮-结瘤的顺序逐级筛选,结合培养观察和回接鉴定。最终获得3株溶磷根瘤菌RS-1,RS-8和RS-14。其中RS-1产生长素能力及回接根瘤固氮酶活性均较高,溶磷量6.98mg·L^-1·d^-1,适应pH 4-12的环境并耐受11%的盐胁迫。表明该筛选方法简便有效,菌株应用前景好。In order to obtain phosphate solubilizing Rhizobiumstrains of sainfoin（Onobrychis viciaefolia）.Winogradsky N-free medium and PKO medium were used to isolate and screen Rhizobiumstrains from sainfoin root nodules.By analyzing the capabilities of sparingly inorganic phosphorus solubilizing,IAA secreting and the nodule nitrogenase activity of screened strains,three rhizobia strains RS-1,RS-8and RS-14 were obtained.And the phosphate solubilizing capability of RS-1strain reached 6.98mg·L^-1·d^-1 in 14 days,and RS-1with higher IAA secreting capability and could survive at pH values ranging from 4to 12,and salt tolerance capability at 11%.Results showed that the screening method used was simple and effective,and the isolated strains could have good prospect of application.

关 键 词：红豆草 根瘤菌 固氮 溶磷 生长素 

分 类 号：S945[农业科学—水产养殖]