七星瓢虫滞育关联基因的转录组学分析  

Transcriptome analysis of diapause-associated genes of Coccinella septempunctata L.

作　　者：齐晓阳 任小云[2] 安涛[2] 陈红印[2] 黄建[1] 张礼生[2] 

机构地区：[1]福建农林大学植物保护学院,农业部闽台作物有害生物综合治理重点实验室,福州350002 [2]中国农业科学院植物保护研究所,中美生物防治实验室,北京100081

出　　处：《环境昆虫学报》2016年第2期238-248,共11页Journal of Environmental Entomology

基　　金：国家自然科学基金(31572062);国家重点基础研究发展计划(973)项目(2013CB127602);公益性行业(农业)科研专项(201103002)

摘　　要：对七星瓢虫正常发育、滞育以及滞育解除的雌成虫进行RNA测序,并对筛选出来的滞育相关基因进行KEGG通路富集分析,从分子水平解析七星瓢虫滞育发机理。本研究以正常发育产卵、滞育30 d以及滞育贮存30 d后解除产卵的七星瓢虫雌成虫为研究对象,分别抽提RNA,合成c DNA,构建c DNA文库,文库检测合格后在Illummina Hiseq 2500测序仪上进行双向测序。根据测序结果,共获取unigene 82820个。采用两两比较法对正常发育组和滞育组、滞育组和滞育解除组进行差异表达分析,分别获得差异表达基因3501个和1427个。深入分析两组比对结果,将在滞育组上调且滞育解除组下调的unigene定义为滞育关联基因,共有443个基因为滞育关联基因。应用KEGG KAAS在线pathway比对分析工具对滞育关联基因进行通路富集分析,结果发现这些基因主要集中在碳水化合物代谢、脂质代谢以及信号转导等途径中。To understand the diapause mechanism of Coccinella septempunctata L. at molecular level,transcriptome sequencing was carried out for female adults in three different states,i. e.,non-diapause（ ND）,diapause（ D） and diapause terminated（ DT）,and KEGG pathway enrichment analysis was performed on the up-regulated expressed genes. In research,total RNA was respectively extracted from the female adults with normal development after oviposition,diapause 30 days,and diapause-terminated after diapause 30 days, and c DNA samples were synthesized to generate c DNA libraries. After quality inspection,two pair sequencing was performed with the Illumina Hi Seq 2500 system. Total of 82820 unigenes were yieled according to sequencing results. When ND and D were compared,3501 differentially expressed genes（ DEGs） were revealed,and 1427 DEGs were detected between D and DT. The DEGs which was highly expressed in the diapause stage compared to non-diapause and diapause-terminated stages was defied as diapause-associated genes. In results,total of 443 unigenes were found to be diapauseassociated genes. The analysis of KEGG enriched pathways were performed on diapause-associated genesusing KAAS（ KEGG Automatic Annotation Server） and found that these genes were mainly involved in carbohydrate metabolism,lipid metabolism and signal transduction.

关 键 词：七星瓢虫 滞育 转录组测序 滞育关联基因 KEGG分析、碳水化合物代谢 

分 类 号：Q963[生物学—昆虫学] S476[农业科学—农业昆虫与害虫防治]