油葵杂交种耐盐性鉴定及幼苗对盐胁迫的生理响应  

Identification and screening of salinity oil sunflower hybrids and effects of salt stress on the physiological characteristics in the seedling stage

作　　者：张艳芳[1] 孙瑞芬[2] 郭树春[2] 侯建华[1] 

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019 [2]内蒙古农牧业科学院,呼和浩特010031

出　　处：《科技导报》2016年第7期94-102,共9页Science & Technology Review

基　　金：内蒙古农牧业创新基金项目(2013CXJJN14)

摘　　要：以油葵杂交种P6、P29、P65、S13和P50为试验材料,采用土培300、350和400 mmol/L 3个不同浓度Na Cl和水培100、120和150 mmol/L 3个不同浓度Na Cl胁迫处理的植株进行形态观察,并对水培120 mmol/L Na Cl胁迫处理第9天的植株进行耐盐性鉴定;水培120和150 mmol/L Na Cl胁迫处理,测定P50和P29的可溶性蛋白含量、Pro含量、MDA含量、SOD活性和POD活性5项耐盐相关生理生化指标,探讨不同油葵杂交种间对盐胁迫的响应差异。结果表明:1)5个油葵杂交种幼苗的耐盐性表现为P50>P65>P6>S13>P29。其中P50和P65的盐害指数分别为0和5.83%,为极强耐盐类型;S13和P6的盐害指数分别为40.28%和51.39%,为中度耐盐类型;P29的盐害指数为80.83%,为盐敏感类型。2)P50和P29品种间比较,5项指标呈现极显著差异;3)同一品种的不同浓度间比较,5项指标呈现极显著差异,不同浓度Na Cl胁迫下,随处理时间的延长,P50的可溶性蛋白含量呈现升高的趋势,P29呈先升后降的趋势;P50和P29的Pro含量、SOD活性和POD活性的变化趋势相似,呈现先升高后降低的趋势;P50和P29的MDA含量呈现升高的趋势。研究表明,盐逆境对油葵幼苗的生长有明显伤害,但P50与P29受到的伤害差异程度达极显著,整体上P50表现出对盐胁迫具有较强抗性。5 oil sunflower hybrids, P6, P29, P65, S13 and P50, are used to study the plant morphologies of the soil culture at 3 different concentrations of NaC1, 300, 350 and 400 mmol/L, and the plant morphologies of the water culture at 3 different concentrations of NaC1, 100, 120 and 150 mmol/L. The tolerance to the salt stress is identified for the water culture with 9 day＇s treatment of 120 mmol/L NaC1. Five physiological and biochemical indexes, including the soluble protein content, the Pro content, the MDA content, the SOD activity, and the POD activity about the salt-tolerance of water cultures of P50 and P29 are determined by two treatments of 120 and 150 mmol/L concentrations of NaC1, to investigate the different responses to the salt stress among oil sunflower hybrids. Results show： 1） The salt tolerance capacities of five oil sunflower hybrids are in the following order： P50 〉 P65 〉 P6 〉 S13 〉 P29. With respect to the degree of salt tolerance, P50 and P65 are very strong salt-tolerant types, S13 and P6 are moderate salt-tolerant types, P29 is a very weak sah-tolerant type. 2） Five indexes show a very significant difference between P50 and P29. 3） Five indexes show very significant difference by comparing with different concentrations of the same species of P29 and P50. P50 shows a strong resistance to the salt stress.

关 键 词：油葵 盐胁迫 耐盐性 生理生化指标 

分 类 号：S565.5[农业科学—作物学]