强骨宝对去势大鼠原发性膝骨关节炎关节软骨影响的实验研究  

Experimental Study on the Effect of Qianggu Bao(强骨宝)on the Articular Cartilage of Primary Knee Osteoarthritis in Ovariectomized Rats

作　　者：王文胜[1] 陈伟[1] 沈保磊 许波[1] 张俐[1] 

机构地区：[1]福建中医药大学,福建福州350122

出　　处：《风湿病与关节炎》2016年第4期10-13,共4页Rheumatism and Arthritis

基　　金：福建省教育厅A类科技项目(JA12167);福建省科技厅资助项目(闽科计〔2016〕10号)

摘　　要：目的:建立去势后原发性膝骨关节炎大鼠模型,观察强骨宝对模型鼠关节软骨形态学及软骨基质的影响,为进一步研究提供基础。方法:1去势后原发性膝骨关节炎模型建立:麻醉下摘除双侧卵巢1周后行疲劳性跑步训练,跑台坡度-15°,频率28 m·min-1,60 min·d-1,每周6 d,建立膝骨关节炎模型。分别于2,4,6周后检测尿液中Ⅱ型胶原羧基端肽(CTX-Ⅱ),制作股骨髁病理切片鉴定模型。2动物分组与给药:将造模成功的大鼠随机分为模型对照组、强骨宝组、阳性对照组,分别给予相应处理。3取材与检测:给药4周后,取尿液、血清、双侧股骨髁,检测尿液中CTX-Ⅱ含量,股骨髁软骨经HE染色处理后行Mankin评分。结果:1模型鉴定:根据尿液CTX-Ⅱ含量及软骨Mankin评分结果,造模4周后关节软骨开始退变,6周后退变明显,模型成功。2给药治疗:Mankin评分及CTX-Ⅱ含量测定结果,与空白对照组比较,模型对照组、强骨宝组、阳性对照组均增高(P<0.01或P<0.05);与模型对照组比较,强骨宝组、阳性对照组均降低(P<0.05);与阳性对照组比较,强骨宝组差异无统计学意义(P>0.05)。结论:强骨宝可减缓原发性膝骨关节炎模型鼠的软骨损伤进程。Objective：To observe the effect of Qianggu Bao（强骨宝）on the articular cartilage of primary knee osteoarthritis in ovariectomized rats.Methods：（1）Establishing models of ovariectomized rats with primary knee osteoarthritis：After anesthesia,bilateral ovariectomy was performed for the experimental rats.After one week,they were forced to do fatigued running training on 15-degree treadmill with frequency of 28 m·min^-1,60 min·d^-1,for 6 days a week to establish knee osteoarthritis models.CTX-Ⅱ in urine was detected respectively after 2,4 and 6 weeks,making pathological identification model of femoral condyle.（2）Grouping and administration：Successfully modeled rats were randomly divided into a model group,a Qianggu Bao group and a positive control group,and each group was given corresponding treatment.（3）Sanjmpling and detection：After 4 weeks of administration,urine,serum and bilateral femoral condyle were sampled to detect the content of CTX-Ⅱ in urine and make Mankin scores to femoral condyle cartilage after HE staining.Results：（1）Model identification：According to the content of CTX-Ⅱ in urine and Mankin scores of femoral condyle cartilage,articular cartilage degeneration began after 4 weeks of modeling,which was obvious after 6 weeks,so the models were successfully established.（2）Administration for treatment：Compared with the blank control group,Mankin score and CTX-Ⅱ content of the model control group,the Qianggu Bao group and thepositive control group all increased（P〈0.01 or P〈0.05）;compared with the model control group,those of the Qianggu Bao group and the positive control group decreased（P〈0.05）;and the difference between the positive control group and the Qianggu Bao group had no statistical significance（P〉0.05）.Conclusion：Qianggu Bao can slow down the process of cartilage damage in model rats with primary knee osteoarthritis.

关 键 词：骨关节炎 膝 强骨宝 动物模型 大鼠 实验研究 

分 类 号：R285.5[医药卫生—中药学]