长春西汀对小鼠血管平滑肌细胞成骨样分化的影响  

Effect of vinpocetine on the osteoblastic differentiation of mouse vascular smooth muscle cells

作　　者：马允允 梁秋华[2] 张正军[2] 孟强[2] 李懂[2] 孙琳[2] 

机构地区：[1]济宁医学院,济宁272067 [2]济宁医学院附属医院,济宁272029

出　　处：《济宁医学院学报》2016年第2期82-86,共5页Journal of Jining Medical University

基　　金：国家自然科学基金资助项目(81400860)

摘　　要：目的初步探讨长春西汀对小鼠血管平滑肌细胞(vascular smooth muscle cells,VSMCs)成骨样分化的影响。方法使用10m Mβ-甘油磷酸钠(beta-glycerophosphate,β-GP)诱导小鼠原代VSMCs向成骨样分化,以10μM长春西汀干预β-GP诱导的小鼠VSMCs,应用Western blotting分析法检测Runt相关转录因子2(Run related transcription factor 2,Runx2),骨形成蛋白-2(bone morphology protein-2,BMP-2)以及核转录因子-Kappa B(nuclear factor kappa B,NF-κB)p65的表达,碱性磷酸酶(alkaline phosphatase,ALP)试剂盒检测ALP活性,茜素红染色观察矿化结节形成的情况。结果 1)β-GP可显著增强ALP的活性,增加Runx2和BMP-2的表达,并促进矿化结节的形成;2)长春西汀可显著减弱ALP活性,抑制Runx2和BMP-2的表达,减少矿化结节的形成;3)长春西汀抑制NF-κB活化。结论长春西汀可通过NF-κB信号通路显著抑制小鼠VSMCs成骨样分化。Objective To investigate the effect of vinpocetine on the osteoblastic differentiation of mouse vascular smooth muscle cells（ VSMCs）. Methods 10 m M of beta-glycerophosphate（ β-GP） was used to induce osteoblastic differentiation of mouse VSMCs. 10μM of vinpocetine was treated on the β-GP-stimulated VSMCs. The protein expression of Run related transcription factor 2（ Runx2）,bone morphology protein-2（ BMP-2） and nuclear factor kappa B（ NF-κB）p65 subunit were determined by Western Blot. Alkaline phosphatase（ ALP） assay kit was used to determine the ALP activity. The formation of mineralized nodules was determined by Alizarin Red S staining. Results 1） β-GP significantly increased ALP activity and the expression of Runx2 and BMP-2 which promoted the formation of mineralized nodules. 2）Vinpocetine significantly decreased ALP activity and the expression of Runx2 and BMP-2,which attenuated the formation of mineralized nodule. 3） Vinpocetine significantly inhibited the translocation of NF-κB p65 into the nucleus. Conclusion Vinpocetine may exert its inhibitory effect on osteoblastic differentiation of VSMCs via NF-κB signaling pathway.

关 键 词：动脉钙化 血管平滑肌细胞 成骨样分化 长春西汀 

分 类 号：R589.9[医药卫生—内分泌]