HPLC-ESI-Q-TOF-MS法分析金钱草颗粒的化学成分  

Analysis of the Chemical Compositions in Jinqiancao Granule by HPLC-ESI-Q-TOF-MS

作　　者：汪丹[1,2] 蔡甜[3] 吴志军[3] 蒋学华[1] 

机构地区：[1]四川大学华西药学院,成都610041 [2]成都大学医学院,成都610106 [3]中国科学院成都生物研究所,成都610041

出　　处：《中国药房》2016年第12期1651-1654,共4页China Pharmacy

基　　金：国家自然科学基金青年项目(No.21305137)

摘　　要：目的：建立分析金钱草颗粒化学成分的方法。方法：采用高效液相色谱-电喷雾-飞行时间质谱（HPLC-ESI-Q-TOF-MS）法。色谱条件：色谱柱为Inertsil ODS-4,流动相为0.1%甲酸-甲醇（梯度洗脱）,流速为0.8 ml/min,柱温为30℃,进样量为15μl;质谱条件：离子源为电喷雾离子原（负离子模式）,端板偏移电压为-500 V,毛细管电泳电压为3 500 V,载气为氦气,雾化和干燥气体为高纯度氮气,流速为6 L/min,压力为1.0 bar,干燥气温度为180℃,扫描范围为50~1 500 m/z。采用Chem Bio Draw Ultra 13.0和Bruker数据分析软件4.0版分析化学成分分子式。结果：从金钱草颗粒中鉴定出27种化学成分,分别为蔗糖、尿苷、没食子酸、新绿原酸、表没食子儿茶素、原儿茶酸、绿原酸、夏佛塔苷、咖啡酸、异夏佛塔苷、香草酸、阿魏酸、金丝桃苷、迷迭香酸、芦丁、异槲皮苷、紫云英苷、槲皮苷、杨梅素、对羟基苯甲酸、槲皮素、柚皮素、木犀草素、山柰酚、异鼠李素、芹菜素、大黄素。结论：咖啡酸、迷迭香酸、香草酸、表没食子儿茶素、尿苷、大黄素均为首次从金钱草制剂中被发现并报道。OBJECTIVE： To establish a method for the analysis of chemical compositions in Jinqiancao granule. METHODS： HPLC-ESI-Q-TOF-MS was adopted. The chromatographic conditions： the column was Inertsil ODS-4 with mobile phase of 0.1% formic acid- methanol （gradient elution） at a flow rate of 0.8 ml/min, column temperature was 30 ℃, and the injection volume was 15 μl. MS conditions： ion source was ESI （negative ion mode）, endplate offset voltage was -500 V, capillary electrophore- sis was 3 500 V, carrier gas was helium gas, atomization and drying gas was high purity nitrogen gas at a flow rate of 6 L/min and a pressure of 1.0 bar, drying air temperature was 180 ℃. Scanning range was 50-1 500 m/z. ChemBioDraw Ultral3.0 and Bruk- er data analysis software 4.0 were used to analyze the chemical composition molecular formula. RESULTS： A total of 27 kinds of chemical components were identified in Jinqiancao granule, involving sucrose, uridine, gallic acid, new chlorogenic acid, （-）-epi- gallocatechin, protocatechuic acid, chlorogenic acid, schaftoside, caffeic acid, schaftoside, vanillc acid, ferulic acid, hyperin, ros- marinic acid, rutin, isoquercitrin, astragalin, quercetin-3-rhamnoside, myricetin, 4-hydroxybenzoic acid, quercetin, naringenin, luteolin, kaempferol, isorhmnetin, apigenin and emodin. CONCLUSIONS： Caffeic acid, rosmarinic acid, vanillic acid, （-）-epigal- locatechin, uridine and emodin are firstly found and reported in the chemical compositions in Jinqiancao granule.

关 键 词：金钱草颗粒 高效液相色谱-电喷雾-飞行时间质谱法 化学成分 

分 类 号：R927[医药卫生—药学]