UPLC法同时测定野生刺五加果和根中5种主要成分的含量  

Simultaneous Determination of 5 Main Components in the Fruits and Root of Wild Acanthopanax senticosus by UPLC

作　　者：姚慧敏[1] 关颖丽[1] 朱俊义[1] 葛延杰 黄媛[1] 张萌[1] 

机构地区：[1]通化师范学院吉林省长白山药用植物研究重点实验室,吉林通化134002 [2]沈阳鑫泰格尔医药科技开发有限公司,沈阳110016

出　　处：《中国药房》2016年第12期1668-1671,共4页China Pharmacy

基　　金：吉林省科技发展计划项目(No.20130206035YY);吉林省教育厅"十二五"科学技术研究项目(No.吉教科合字〔2012〕第357号);吉林省大学生创业训练计划项目(No.thsys1061)

摘　　要：目的：建立同时测定野生刺五加果和根中5种主要成分紫丁香苷、绿原酸、刺五加苷E、异嗪皮啶和槲皮素-3-鼠李糖苷含量的方法。方法：采用超高效液相色谱法。色谱柱为Waters ACQUITY UPLC HSS T3,流动相为乙腈-0.3%磷酸（梯度洗脱）,流速为0.2 ml/min,检测波长为300 nm,柱温为30℃,进样量为10μl。结果：紫丁香苷、绿原酸、刺五加苷E、异嗪皮啶、槲皮素-3-鼠李糖苷检测质量浓度线性范围分别为24.56~184.2μg/ml（r=0.999 3）、18.454~138.405μg/ml（r=0.999 3）、8.416~63.12μg/ml（r=0.999 7）、3.286~24.645μg/ml（r=0.999 3）、2.522~18.915μg/ml（r=0.999 8）;精密度、稳定性、重复性试验的RSD〈1%。刺五加果中上述5种成分的加样回收率分别为99.14%~100.50%（RSD=0.48%,n=6）、99.03%~100.45%（RSD=0.50%,n=6）、99.22%~100.44%（RSD=0.44%,n=6）、99.80%~100.80%（RSD=0.44%,n=6）、99.76%~101.10%（RSD=0.51%,n=6）;刺五加根中上述前4种成分的加样回收率分别为99.21%~101.20%（RSD=0.73%,n=6）、99.81%~101.20%（RSD=0.52%,n=6）、100.00%~101.80%（RSD=0.62%,n=6）、99.22%~100.40%（RSD=0.47%,n=6）。结论：该方法操作简便、稳定、重复性好,可用于野生刺五加果和根中紫丁香苷、绿原酸、刺五加苷E、异嗪皮啶和槲皮素-3-鼠李糖苷含量的同时测定。OBJECTIVE：To establish a method for the 5 main components （original syringin, chlorogenic acid, eleutheroside E, isofraxidin and quercetin-3-rhamnoside） in the fruits and roots of wild Acanthopanax senticosus. METHODS： UPLC was per- formed on the colunm of Waters ACQUITY UPLC HSS T3 with mobile phase of acetonitrile-0.3% phosphoric acid （gradient elu- tion） at a flow rate of 0.2 ml/min. Detection wavelength was 300 rim, column temperature was 30 ℃, and injection volume was 10 μl. RESULTS： The linear range was 24.56-184.2 μg/ml for syringin （r=0.999 3）, 18.454-138.405 gg/ml for chlorogenic acid （r= 0.999 3）, 8.416-63.12 gg/ml for eleutheroside E （r= 0.999 7）, 3.286-24.645 μg/ml for isofraxidin （r= 0.999 3） and 2.522-18.915 gg/ml for quercetin-3-rhamnoside （r= 0.999 8） ; RSDs of precision, stability and reproducibility tests were lower than 1% ; recover- ies were 99.14%-100.50% （RSD=0.48% ,n=6）for syringing in the fruits ofA. senticosus,99.03%-lO0.45% （RSD=0.50% ,n=6） for chlorogenic acid in the fruits ofA. senticosus,99.22%-100.44% （RSD=0.44% ,n=6）for eleutheroside E in the fruits of A. sen- ticosus,99.80%-100.80%（RDS=0.44% ,n=6）for isofraxidin in the fruits ofA. senticosus, 99.76%-101.10% （RSD=0.51% ,n=6） for quercetin-3-rhamnoside in the fruits ofA. senticosus;99.21%-101.20%（RSD=0.73% ,n=6） for syringing in the root of A. senti- cosus,99.81%-101.20% （RSD=0.52% ,n=6） for chlorogenic acid in the root ofA. senticosus, 100.00%-101.50% （RSD=0.62%, n=6）for eleutheroside E in the root ofA. senticosus,99.22%-100.40% （RSD=0.47% ,n=6）for isofraxidin in the root of A. senti- cosus. CONCLUSIONS：The method is simple and stable with good reproducibility, and can be used for the simultaneous determi- nation of original syringin, chlorogenic acid, eleutheroside E, isofraxidin and quercetin-3-rhamnoside in the fruits and root of wild A. senticosus.

关 键 词：刺五加果 刺五加根 超高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]