低温胁迫对4种绿化幼苗生理的影响  

Effect of low temperature stress on physiological characteristics of seedlings of four landscape species

作　　者：王卓敏[1] 周彤彤[1] 薛立[1] 许建新 

机构地区：[1]华南农业大学林学与风景园林学院,广州510642 [2]深圳市铁汉生态环境股份有限公司,深圳518040

出　　处：《安徽农业大学学报》2016年第2期209-214,共6页Journal of Anhui Agricultural University

基　　金：深圳市战略性新兴产业专项资金项目(GCZX20120618100801416)资助

摘　　要：以花旗木Cassia bakeriana、海南蒲桃Syzygium hainanense、铁冬青Ilex rotunda和紫楠Phoebe sheareri4种幼苗为试验材料,利用人工气候箱模拟低温胁迫环境,对幼苗进行0 d、2 d、4 d和6 d的6℃的低温处理及自然状态下恢复2 d后,测定幼苗的生理指标,最后用主成分分析法对其抗寒性进行综合评定。结果表明,随着胁迫时间的延长,花旗木的叶绿素含量持续增加,海南蒲桃先升后降,铁冬青先降后升,紫楠呈现降升降的变化;花旗木和铁冬青的可溶性糖含量趋于上升,海南蒲桃上升后波动,而紫楠先升后降;花旗木、海南蒲桃和铁冬青幼苗的可溶性蛋白含量持续上升,紫楠小幅变化;海南蒲桃、花旗木和铁冬青的脯氨酸含量持续下降,紫楠上升后保持稳定;海南蒲桃、花旗木、铁冬青和紫楠的SOD活性在低温胁迫2 d后上升到最大值;海南蒲桃、花旗木和铁冬青的MDA含量先升后降,紫楠则小幅波动。主成分分析表明,低温下幼苗的抗寒能力为花旗木>紫楠>铁冬青>海南蒲桃。Physiological characteristics of seedlings of Cassia bakeriana, Phoebe sheareri, Ilex rotunda and Syzygium hainanense were studied after they were exposed to a low temperature stress（6℃） in the Artificial Climate Box. After continuous treatments at 6℃ for 0, 2, 4 and 6 days and then a recovery period of 2 days in the nature, the physiological indexes of the seedlings were determined and their cold resistance was evaluated using principal component analysis. The results showed that with increasing low temperature stress time, the chlorophyll content of C. bakeriana continuously increased, and that of S. hainanense seedlings increased followed by a decrease. In I. rotunda, chlorophyll decreased followed by an increase, whereas in P. sheareri, it decreased followed by an increase and then decreased. The soluble sugar contents in C. bakeriana and I. rotunda increased gradually, and that of S. hainanense seedlings increased and fluctuated, whereas in P. sheareri seedlings, soluble sugar increased followed by a decrease. The soluble protein contents in C. bakeriana, S. hainanense and I. rotunda continuously increased, and in P. sheareri it slightly changed. The proline contents in C. bakeriana, S. hainanense and I. rotunda continuously decreased, while that in P. sheareri increased and kept steady. The SOD activity in the seedlings of four species reached their maximum levels after a low temperature stress for 2 days. The MDA contents of C. bakeriana, S. hainanense and I. rotunda increased followed by a decrease and that in P. sheareri changed slightly. Principal component analysis indicated that the cold resistance ability of the seedlings of four species decreased in the order of C. bakeriana〉 P. sheareri〉 I. rotunda〉 S. hainanense.

关 键 词：幼苗 低温胁迫 生理指标 主成分分析 

分 类 号：S731[农业科学—林学]