水稻端粒长度测定及其遗传特性研究  

Measurement of Telomeric Length and Hereditary Character of Oryza Sativa

作　　者：叶聪莹 黄倩[1] 马国兴[1] 林高强[1] 曹佳薇[1] 刘小川[1] 

机构地区：[1]浙江理工大学生命科学学院

出　　处：《浙江理工大学学报（自然科学版）》2016年第3期463-468,共6页Journal of Zhejiang Sci-Tech University(Natural Sciences)

摘　　要：端粒在保护和维持染色体完整性方面发挥了重要作用，且与细胞衰老、癌变密切相关。由于同一细胞内，不同染色体端粒长度存在差异，研究单个端粒长度的遗传特性对于深入了解端粒的作用，阐明端粒与细胞周期间的关系具有重要意义。本研究利用水稻籼亚种和粳亚种之间平均端粒长度存在明显差异的特点，结合它们的杂种F1、F2代群体，采用改良的单个端粒长度测定技术（STELA），对T02p、T02q、T04q、T09p、T10q、T11p等6个端粒长度进行了分析。结果表明：单个端粒长度与相关染色体长度存在相关性，且具有数量性状遗传特征。更重要是F1代与亲本之间有5个端粒没有显著差异，仅有T04q一个端粒呈现出超显性遗传效应，且超过极显著的水平。因此，推测调控细胞周期的端粒长度并非是整个细胞平均端粒长度，而是某些或极少数关键端粒的长度。Telomere performs an important function of protecting and maintaining chromosome integrity and is closely related to cell senescence and cancerization.Since there are differences in lengths of different chromosome telomere within a cell,to study the hereditary character of the length of a single telomere in cells could help to further understand telomere functions and illuminates the relation between the telomere and cell cycle.In this study,according to the significantly different average telomere lengths of rice indica and japonica subspecies,in combination with hybrid F1 and F2generation groups,analyses on the telomere lengths of T02 p,T02q,T04 q,T09p,T10 q and T11 p were carried by using the improved single telomere length analysis(STELA).The results show that the single telomere length is correlated to the relevant chromosome length and is characterized by quantitative character inheritance.What’s more,between F1 hybrid and the parents,there was no significant difference in 5telomeres except T04 q,which shows over-dominance inheritance and highly significantly exceeds t he level.Therefore,it is presumed that the telomere length of cell cycle is not the average telomere length of the whole cell but the length of some or very few telomeres are regulated.

关 键 词：水稻 端粒长度 STELA 遗传 

分 类 号：Q943[生物学—植物学]