水稻迟抽穗突变体dth9的遗传分析与基因定位  

Genetic Analysis and Gene Mapping of a Heading-delayed Mutant dth9 in Rice(Oryza sativa L.)

作　　者：叶卫军[1,2] 胡时开[2,3] 吴立文[2] 郭龙彪[2] 钱前[2,3] 

机构地区：[1]浙江大学农业与生物技术学院,杭州310058 [2]中国水稻研究所,杭州310006 [3]中国农业科学院深圳农业基因组研究所,广东深圳518120

出　　处：《中国水稻科学》2016年第3期232-238,共7页Chinese Journal of Rice Science

基　　金：国家自然科学基金资助项目(31501279;31521064;31271700);国家973计划资助项目(2013CBA01405);中国博士后科学基金资助项目(2015M570181);深圳市科技计划资助项目(JCYJ20150630165133402)

摘　　要：在EMS诱变的93-11突变体库中筛选到一个稳定遗传的迟抽穗突变体dth9(days to heading 9)。该突变体的抽穗期比野生型延长了50d左右,其他农艺性状基本无异。遗传分析表明迟抽穗性状受一个隐性核基因控制。以突变体dth9与日本晴和武运粳7号杂交构建的F2分离群体作为定位群体,利用SSR标记和新开发的8个InDel标记,将DTH9定位在第9染色体着丝粒附近D9-9和D9-17之间240kb的区间内,该区域尚未发现与抽穗期有关的基因。此外,实时荧光定量PCR结果表明,在突变体dth9中与抽穗期相关基因的表达量显著降低。A heading-delayed mutant,dth9(days to heading 9)was identified from an ethyl methylsulfonate(EMS)-induced 93-11 mutant library.The heading date of dth9 delayed about 50 days compared to the wild-type and there was no significant difference in other agronomic traits.Genetic analysis showed that the phenotype of dth9 was controlled by a single recessive nuclear gene.To map this gene,two F2 populations were generated by crossing the dth9 mutant with Nipponbare or Wuyunjing 7as mapping populations.By using SSR markers and eight new designed InDel markers,DTH9 was narrowed to a 240 kb interval between the markers D9-9 and D9-17 near the centromere of chromosome 9,there were no reports about genes associated with heading date in this interval.In addition,the expression levels of genes related to heading date were significantly decreased in dth9 by quantitative real-time PCR analysis.

关 键 词：水稻 抽穗期 遗传分析 基因定位 

分 类 号：S511[农业科学—作物学]